You purify a protein from an E. coli lysate using affinity chromatography and run fractions from the column on an SDS-PAGE. a. What does each lane on the gel show (e.g. elution, flowthrough, marker)? Lane M: ______________ Lanes 1-2: ______________ Lanes 3-5: ______________ b. What is the approximate size of the target protein? ______________
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Related Questions
PLS HELP I DONT UNDERSTAND!! BRAINLIEST + 25 POINTS IMAGE BELOW!!
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is it important to have circulatory system for single cell or multicellular organism?
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A single gene with two alleles control variation in haemoglobin type in sheep. There are three different phenotypes corresponding to genotypes S^AS^A S^AS^B and S^BS^B respectively in a flock of 175 sheep the frequency of allele S^A was found to be 0.6 and frequency of allele S^B WAS found to be 0.4
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Populations in Hardy-Weinberg equilibrium express the same allelic and genotypic frequencies through generations. Number of individuals expressing each phenotype: 63 sheep S^AS^A, 84 sheep S^AS^B, 28 sheep S^BS^B.
What is Hardy-Weinberg equilibrium?The Hardy-Weinberg equilibrium theory states that allelic and genotypic frequencies in populations that is in equilibrium remain the same through generations. No evolutive forces are acting on these populations, so no evolution occurs.
Assuming a diallelic gene,
Allelic frequencies
The frequency of the dominant allele f(X) is pThe frequency of the recessive allele f(x) is qGenotypic frequencies
p² (Homozygous dominant genotypic frequency), 2pq (Heterozygous genotypic frequency), q² (Homozygous recessive genotypic frequency).The addition of the allelic frequencies equals 1
p + q = 1.
The sum of genotypic frequencies equals 1
p² + 2pq + q² = 1
In the exposed example,
Alleles Frequencies
S^A f(S^A) = p = 0.6S^B f(S^B) = p = 0.4Genotypes
S^AS^A ⇒ homozygousS^AS^B ⇒ heterozygousS^BS^B ⇒ homozygousWe need to get the number of animals expressing each genotype and phenotype. To do it, we just need to use allelic frequencies and calculate genotypic frequencies (p², 2pq, and q²), and then multiply these values by the total number of animals (N = 175).
Genotypic and Phenotypic frequencies
F(S^AS^A) = p² = 0.6² = 0.36
F(S^AS^B) = 2pq = 2 x 0.6 x 0.4 = 0.48
F(S^BS^B) = q² = 0.4² = 0.16
Number of individuals expressing each phenotype
S^AS^A ⇒ 0.36 x 175 = 63 individualsS^AS^B ⇒ 0.48 x 175 = 84 individualsS^BS^B ⇒ 0.16 x 175 = 28 individualsYou can learn more about the Hardy-Weinberg equilibrium at
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Complete question
A single gene with two alleles control variation in haemoglobin type in sheep. There are three different phenotypes corresponding to genotypes
S^AS^A S^AS^B and S^BS^BIn a flock of 175 sheep the frequency of allele S^A was found to be 0.6 and frequency of allele S^B WAS found to be 0.4.
Using the hardy weinberg equation calculate the number of sheep with each phenotypes in the flock show your working
A control variable is: A. Measured to show the effect of a change. B. Collected to draw conclusions. C. Changed to test a hypothesis. D. Kept the same to make an experiment a fair test.
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‘a controlled variable’
A control variable Kept the same to make an experiment a fair test. Thus, option D is correct.
What Is a Control Variable ?
Variables may be controlled directly or indirectly by randomization methods.
A control variable is a type of variable unit which held constant in a research study.
It’s variability does not involve study aims but it is controlled and also show an influencing effect on the study outcomes.
It limits the effect of confounding and other extraneous variables and enhance the internal validity of a study.
In an experiment, the effect of an independent variable on a dependent variable can be investigated, for this control variables will help to understand that the results are caused by manipulated data in experiments.
Thus, option D is correct.
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n
3
Read the excerpt from Jane Eyre by Charlotte Brontë.
My head still ached and bled with the blow and fall I
had received: no one had reproved John for wantonly
striking me; and because I had turned against him to
avert farther irrational violence, I was loaded with
general opprobrium.
"Unjust!-unjust!" said my reason, forced by the
agonising stimulus into precocious though transitory
power.
What does John's attack of Jane most likely represent
about the human condition?
O cruelty toward the defenseless
O conflict between family members
O the victory of emotion over reason
O the power of the rich over the poor
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The thing which John's attack on Jane most likely represents about the human condition is C. the victory of emotion over reason
What is a Narration?This refers to the use of a narrator to show the sequence of events and how they occurred in a given plot.
Hence, we can see that from the given narration, there is a description of the attack which the narrator had faced from John and how when she wanted to retaliate, her reason tried to stop her from engaging in further violence.
This shows the victory of emotion over reason as she did strike back when her emotions goaded her into taking action by stating that it was a shameful thing.
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The cells in the cardiac muscle of an animal's heart have branches that form
a network. What does this feature enable the cardiac muscle tissue to do?
OA. Contract all at once in order to make the heart beat
B. Provide support to the soft tissues in the heart to give it its shape
OC. Form a strong, protective barrier around the heart
D. Store energy that the heart needs to keep beating
SUBMIT
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Answer:A
Explanation:
Which statement correctly describes what proteins, lipids, and glycogen have in common
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Answer:
They are all used as primary energy sources during exercise. They are each stored in the liver to be used as a ready energy reserve. They each can be broken down to provide ATP to cells.
Answer:
The statement that correctly describes what proteins, lipids, and glycogen have in common is C. they each can be broken down to provide ATP to cells. Proteins, lipids, and glycogen all consist of a group of molecules, which means they can be broken down to these smallest items. ATP, or adenosine triphosphate, is a molecule that can be found in anything, and which we need for everything because it is a source of energy in living beings.
Explanation:
Which fact supports the following conclusion?
Public opinion caused the agriculture industry to rethink animal treatment.
O Organic food sales have been steadily rising since the 1990s.
O Livestock is often housed inside with regular periods of outdoor exercise.
O The demand for free-range and cage-free products has increased.
O Consumers are concerned about the health risks of genetic modification.
Answers
Answer:
hello i think b not completely sure
Explanation:
Answer:The demand for free-range and cage-free products has increased.
Explanation: Did it on Edge
If there are 20 individuals in a population, the genotypes of the population in generation 1 are as follows AA=1, AB=13, BB=6. After 10 generations are AA=5,AB=12 and BB=3. What can be said about the population?
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The population of AA increases but on the other hand AB and BB the population decreases after 10th generation.
What is genotype?The genetic makeup of an individual is called their genotype. The two alleles that a person has inherited for a single gene are referred to by this phrase in more detail.
Here for Generation 1, AA = 1, AB = 13, BB = 6
After 10 years it changes AA = 5,AB = 12 and BB =3.
Because AA genotype is generate new genes and alleles, increasing genetic variety In a population, new genetic diversity may be formed within generations, hence a population with a fast rate of reproduction would most likely have a high level of genetic variety.
On the other hand, for AB and BB is decrease in no because:
Rare alleles can disappear due to genetic drift, which can also shrink the gene pool. According to the theory that genetic drift contributes to the development of new species, genetic drift can also result in a new population being genetically diverse from its ancestral population.
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In a population recovering from the Bottleneck Effect, the initial gene pool is likely to be as genetically diverse as the original populatio
True or False
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Answer: I’d say True
Explanation: Genetic drift can result in the loss of rare alleles, and can decrease the size of the gene pool. Genetic drift can also cause a new population to be genetically distinct from its original population, which has led to the hypothesis that genetic drift plays a role in the evolution of new species.
PLEASE HELP!!
Name three organelles that you expect to be abundant in the firefly cells based on the organelle function AND why?
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Answer: Mitochondria, Ribosomes, and the Golgi Apparatus
Explanation:
Mitochondria because the cells need a lot of energy to produce light. Ribosomes, because the cells need them to synthesis more proteins to allow them to survive longer. The Golgi Apparatus, because the cells will need more proteins so the Ribosomes can allow the cell to survive longer.
Plsssssssss helpppppp
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Considering the simulation of antibiotic resistance: briefly explain what happens to a diverse population of bacteria if they are treated repeatedly with the same antibiotic and not all of them die. What was the population's antibiotic resistance level before being exposed to the antibiotic (1 sentence)? What happened to the “good” and antibiotic-resistant bacteria when they were exposed to the antibiotic (1-3 sentences)? What was the population's antibiotic resistance level like after antibiotic treatment (1 sentence)?
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Answer: A diverse population of bacteria that is treated repeatedly with the same antibiotic and not all of them die will eventually lead to the emergence of antibiotic-resistant bacteria that will thrive and become dominant. The population's antibiotic resistance level before being exposed to the antibiotic was low or non-existent. The "good" bacteria will die while the antibiotic-resistant bacteria will survive and reproduce. After antibiotic treatment, the population's antibiotic resistance level will be higher than before.
Explanation:
what would a low number of oxygen bubbles indicate about photosynthesis
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A low number of oxygen bubbles indicates the rate of photosynthesis.
What is Photosynthesis?This is the process in which green plants manufacture their food. Carbon dioxide is used up and oxygen is given off during this process.
The bubbles means oxygen is present which translates to a low number of bubbles depicting a decrease in photosynthetic activity and vice versa.
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1. What conclusions can you draw about how structural changes to genes (mutations) affect proteins and may result in harmful, beneficial, or neutral effects to the structure and function of the organism? Write an evidence-based claim that cites evidence from the results of the paper airplane test as a model.
4.12 Lab: Mutations
100 POINTS TO WHOEVER ANSWERS!
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Answer:
Proteins are molecules that consist of a long chain of amino acid sequences. Changes or mutations in this molecules causes misfolding resulting in different structure. As we can tell from the experiment these changes in the way you fold the airplane changed the effectiveness of the flight some traveled further some traveled less. So we can conclude that changes in gene structure can have either a positive or negative effect on the organism.
Explanation:
I had to do the same lab hope you get a good grade on it!
mention 5 signs and symptoms of HIV positive devolop to AIDS
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Answer:
Fever.
Chills.
Rash.
Night sweats.
Muscle aches.
Sore throat.
Fatigue.
Swollen lymph nodes.
etc
hope you like it and useful
Question 2 of 10
You're a nurse in the emergency room of a hospital. Which of the following
patients should you prioritize first?
O A. Padma sprained her ankle.
B. Mary is having an asthma attack and cannot breathe.
C. Sonya was in a car accident and has a chipped tooth.
D. Ronald's back hurts after he fell off his bike.
SUBMIT
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Answer:
Mary, who is having an asthma attack and cannot breathe, should be prioritized first as she is in immediate danger and requires urgent medical attention.
Explanation:
The graph shows the population growth of a colony of insects over time.
Which statement below best explains what might have occurred to the population of insects at point A?
A
The health of the insects improved, causing the population to stabilize.
B
A predator was introduced, causing a decline in population growth.
C
The population growth decreased as individual insects aged.
D
The population reached a carrying capacity and stabilized.
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Answer: The population reached a carrying capacity and stabilized.
Answer:
the population stabilized
Explanation:
because it continues going up, not down
Doctors sometimes use a vaccine to prepare the body to defend itself against future infections. These vaccines most often containanswer choicesantibodiesantibioticswhite blood cellsweakened pathogens
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Doctors sometimes use a vaccine to prepare the body to defend itself against future infections.
These vaccines most often contain weakened pathogens.
A vaccination is a biological treatment that boosts resistance to a specific disease. It includes a pathogen that doesn't cause disease, like a virus or pathogens that has been weakened or rendered inactive. The immune system responds to the introduction of this virus by creating specific antibodies in response to it because it perceives it as a foreign substance. The immune system is trained to recognise and respond promptly and effectively if the person is exposed to the real virus later on through this process of exposure to a harmless variant of the disease. White blood cells, antibiotics, and antibodies are all parts of the immune system, but they are not usually included in vaccinations because they are not required to develop a protective immunity.
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Sophia accidentally steps on a nail. She reflexively pulls her foot up. This movement was done without the involvement of _____.
the brain
nerves
the spinal cord
muscles
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Sophia accidentally steps on a nail. She reflexively pulls her foot up. This movement was done without the involvement of _____. it's the brain
Classify the silkmoth
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Answer:
Bombyx mori, the domestic silk moth, is an insect from the moth family Bombycidae. It is the closest relative of Bombyx mandarina, the wild silk moth. The silkworm is the larva or caterpillar of a silk moth.
Explanation:
Silkworm, common name for the silk-producing larvae of any of several species of moths. Silkworms possess a pair of specially modified salivary glands called silk glands, or sericteries, which are used in the production of cocoons. The silk glands secrete a clear, viscous fluid that is forced through openings, called spinnerets, on the mouthparts of the larva; the fluid hardens as it comes into contact with air. The diameter of the spinneret determines the thickness of the silk thread produced.
The best-known silkworm is the larvae of the common, domesticated silkworm moth. This moth, native to China, was introduced into Europe and western Asia in the 6th century AD and into North America in the 18th century. The moth has been cultivated for many centuries and is no longer known in the wild state. Breeders have produced many varieties of the moth, the most important of which produce three broods of young annually.
A typical adult silkworm moth is yellow or yellowish-white, with a thick, hairy body, and has a wingspread of about 3.8 cm (about 1.5 in). The adult has rudimentary mouthparts and does not eat during the short period of its mature existence; the female dies almost immediately after depositing the eggs, and the male lives only a short time thereafter. The female deposits 300 to 400 bluish eggs at a time; the eggs are fastened to a flat surface by a gummy substance secreted by the female. The larvae, which hatch in about ten days, are about 0.6 cm (about 0.25 in) long. The larvae feed on leaves of white mulberry, Osage orange, or lettuce. Silkworm caterpillars that are fed mulberry leaves produce the finest quality silk. Mature larvae are about 7.5 cm (about 3 in) long and yellowish-gray or dark gray in color.
About six weeks after hatching, the common silkworm stops eating and spins its cocoon. The length of the individual fiber composing the cocoon varies from 300 to 900 m (1000 to 3000 ft). The silkworm pupates for about two weeks; if allowed to complete its pupation period, it emerges as an adult moth. Tearing during emergence damages the silken cocoon beyond commercial use. Therefore, in the commercial production of silk, only enough adult moths are allowed to emerge to ensure continuation of the species. Most of the silkworms are killed by heat, either by immersion in boiling water or by drying in ovens.
Other moths known as silkworm moths include the giant silkworm moths. The larvae of these large moths also spin silken cocoons, but they are less widely used for commercial silk production.
Scientific classification: Silkworms belong to the order Lepidoptera. The domesticated silkworm moth makes up the family Bombycidae and is classified as Bombyx mori. The giant silkworm moths make up the family Saturniidae.
the value of a reflex angle lies between
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Answer:
reflex angle is an angle that measures more than 180 degrees and less than 360 degrees
Answer:
more then 180 degree and less then 360 degree
6. Which generations saw a population altering incident? Use your imagination to invent a
possible environmental incident that could have caused this shift in populations.
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Answer:
Generations 1 and 6.
Some ideas for incidents might include a forest fire, lack of snowfall, flooding, earthquake, etc.
Explanation:
(:
Which is a biotic factor?
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Answer:
a living organism that shapes its environment
Explanation:
hope this helps
Which of the following describes an acquired trait? *
1 point
An ant has six legs.
A rose has red petals.
A boy dyed his hair blue.
A dog has brown fur.
HELP ME PLESE ANYONE HELPP
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What is the test variable in science
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Answer:
Anything that can change
Explanation:
write a letter to your uncle who is in abroad telling him u want to join vacation classes with ur friends but ur father is facing some financial problems so he can't afford so u need the help of your uncle who is in abroad
Answers
Explanation:
Dear Uncle [Uncle's Name],
I hope this letter finds you in good health and high spirits. I have been meaning to share some news with you, and I believe your guidance and support can make a significant difference in my life.
As you know, the summer vacation is fast approaching, and my friends and I have been planning to join vacation classes together. It is an opportunity for us to learn and engage in activities that would enhance our skills and broaden our horizons. We are all excited about the prospect of spending quality time together while acquiring new knowledge.
However, I regret to inform you that my father is currently facing some financial difficulties. Due to unexpected circumstances, he is unable to afford the expenses associated with the vacation classes. It has been a challenging time for our family, and my father's priority is to ensure our basic needs are met.
In light of these circumstances, I am writing to you, dear uncle, with the hope that you may consider extending your support. Your assistance would mean the world to me and enable me to participate in these vacation classes alongside my friends. Your generosity has always been a pillar of strength for our family, and I am sincerely grateful for everything you have done for us.
Attending these classes would not only be a source of knowledge and skill development but also a chance for me to bond with my friends and create lasting memories. It would enhance my educational journey and contribute to my personal growth. I genuinely believe that this opportunity would be a valuable investment in my future.
I understand that everyone has their own financial responsibilities and commitments. Therefore, I completely respect any decision you make, and I am grateful for your consideration. If it is not feasible for you to provide financial assistance at this time, I completely understand, and I will continue to explore other alternatives.
Thank you, dear uncle, for taking the time to read my letter. I eagerly await your response and value your guidance and support. Regardless of the outcome, please know that your love and presence in our lives mean more to me than anything material.
Sending my warmest regards and affection to you and the family. Take care, and I hope to hear from you soon.
With heartfelt gratitude,
[Your Name]
HELP ASAP
Describe one way an organism can grow other than increasing in size
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Answer and Explanation: Growth can be classified into two categories in living organisms. It is vegetative and reproductive growth.
Biologically, other than growing in size, any organism will show reproductive growth. Be it a plant, animal, fungus, or any other microorganism, all of them show reproductive growth.
In the biological world, there are four main types of reproductive growth. They are vegetative reproduction, asexual reproduction, sexual reproduction, and parthenogenesis. Like size growth, reproductive growth can also be logarithmic, or exponential.
Hence, reproductive growth is another very important form of growth other than the size of the body. Reproduction ensures the continuity of the generation and maintenance of species. Reproductive growth also helps to bring in genetic variation and ensure survival due to mutation.
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Using the concept of natural selection explain why giraffes have such long necks
Answers
The giraffe's neck, which may grow to be two metres long, was chosen because it gives the giraffe exclusive access to the tree's topmost leaves, which no other animal can reach.
Thus to survive and avoid competition for food, giraffe neck adapted to feed on different parts of the tree.
Glucose, one of the products of photosynthesis
Question 2 options:
stores excess nuclear energy within each of the atoms within the molecule
stores chemical potential energy within the bonds of the molecule
stores chemical potential energy within the nucleus of each atom within the molecule
stores thermal energy as the heat from the sun is required to form it
Answers
Answer:
stores chemical potential energy within the bonds of the molecule
Explanation:
Glucose, which has the chemical formula, C6H12O6, is the major product of photosynthesis. It is the way through which autotrophic organisms store energy. Glucose is an organic molecule capable of storing chemical potential energy in its chemical bonds. The photosynthetic equation is:
6CO2 + 6H2O → C6H12O6 + 6O2
However, this potential energy is released and made active when glucose molecule is broken down and converted to ATP via cellular respiration.