What bonds does the denaturation step of pcr break?.

Answers

Answer 1

The denaturation step of PCR breaks the hydrogen bonds between DNA strands. PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA sequences.

The process involves a series of temperature cycles, and one of the key steps is denaturation. During denaturation, the DNA template is exposed to high temperatures, typically around 94-98°C, causing the hydrogen bonds between the two DNA strands to break. DNA is a double-stranded molecule consisting of two complementary strands held together by hydrogen bonds between the nitrogenous bases. The denaturation step raises the temperature beyond the DNA's melting point, causing the hydrogen bonds to weaken and eventually break. As a result, the two DNA strands separate, forming two single-stranded templates. Breaking the hydrogen bonds during denaturation is essential because it allows the subsequent steps of PCR, such as annealing and extension, to occur. In the annealing step, DNA primers bind to the single-stranded templates, providing a starting point for DNA synthesis. The extension step involves the addition of nucleotides by DNA polymerase to extend the primers and replicate the target DNA sequence.

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Related Questions

General description about general biology 2 in Grade 12 subjects

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General Biology 2 is a subject typically taught in Grade 12 as part of the high school curriculum. It is designed to provide students with a deeper understanding of biological concepts and principles building upon the foundational knowledge gained in General Biology 1 or introductory biology courses.

General Biology 2 focuses on various topics related to living organisms and their interactions with the environment. The subject typically covers more advanced concepts in areas such as genetics, evolution, ecology, and human biology. Students delve into the intricacies of cellular processes, molecular genetics, inheritance patterns, population dynamics, and the interrelationships between organisms and their ecosystems.

The course often includes both theoretical knowledge and practical applications. Students may engage in laboratory experiments, data analysis, and scientific inquiry to reinforce their understanding of biological concepts. They may also explore case studies and real-life examples to examine the relevance of biology in everyday life and current scientific advancements.

General Biology 2 aims to enhance students' critical thinking, problem-solving, and analytical skills, as they learn to apply biological principles to real-world scenarios. The subject provides a foundation for further studies in biology, life sciences, or related fields at the college or university level.

Overall, General Biology 2 in Grade 12 serves as a continuation of the exploration of the fundamental principles of biology, fostering a deeper understanding of the complexities of life and the natural world. It prepares students for advanced studies in biology and helps them develop a broader perspective on the diversity and interconnectedness of living organisms.

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what are the characteristics of fungi​??

Answers

Answer:

Some of the characteristics of fungi are it is a

- unicellular and multicellular

-  has protective cell wall

-  doesn't do photosynthesis

- has a nucleus to store DNA

9. Approximately how long has a person been dead if his/her body temperature is 91.6°F?

Answers

Answer:

7 hours

Explanation:

what do i have to draw?

what do i have to draw?

Answers

The afflicted man and the purebred female are depicted in a pedigree chart that displays the potential genotypes of their progeny.

A pedigree reveals the ties between members of a family and identifies those who share certain genetic pathogenic features, illnesses, and vital position with other members of the family. A pedigree can be used to determine how illnesses are passed down within a family.

A pedigree animal is thought to be of high quality since it is derived from animals that were all the same breed.

The phrase is frequently used to describe a purebred dog's pedigree in the animal world.

The image of the pedigree is attached below.

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Describe the offspring of a true-bred white flowering pea plant

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The offspring will have the same traits. Therefore it will be white.

The offspring of true-bred white-flowering pea plants have the same traits. So, they are white in color.

What is offspring?

Offspring is the product of reproduction. It acquires the characters of parents and consists of some variations as well.

While performing a cross between pure red and pure white, pink flowers are obtained in the F1 generation due to incomplete dominance. Now, on selfing, this F1 generation, red, pink, and white flowers are produced in the ratio of 1:2:1, where true-bred white flowers are obtained.

Therefore, the offsprings of a true-bred white flowering have the same traits, and they are recessive in nature.

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How far () would the muon travel before it decayed, if there were no time dilation?

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The muon travel before it decayed, if there were no time dilation is approximately 92.4 m

The muon is a subatomic particle that only lasts for 2.2 μs. However, if there were no time dilation, it would be possible to calculate how far it could travel before decaying. In order to do so, one would need to know the muon's speed, which can be found using the Lorentz factor formula:γ = 1 / sqrt(1 - (v/c)^2)where v is the muon's speed and c is the speed of light.

Once the speed is found, the distance it can travel is simply the product of its speed and its lifetime:distance = speed x lifetime

For a muon travelling at 0.99c, its Lorentz factor would be 7.09.

Therefore, its speed would be v = 0.99c / 7.09 = 0.14c.

Using its lifetime of 2.2 μs, the distance it can travel is:distance = 0.14c x 2.2 μs = 92.4 m. In conclusion, if there were no time dilation, a muon travelling at 0.99c would travel approximately 92.4 m before decaying.

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which of the following is true about the Earth’s layers? A The asthenosphere is the thickest of Earth’s layers. B All of the layers are liquid and extremely hot. C There is little to no pressure on the solid core. D The Earth’s core has two different parts.

Answers

Answer:

D

Explanation:

The Earth’s core has two different parts is true about the earth's layer.

What is Earths core?

The extremely hot and dense core of our planet is called the Earth's core. Underneath the chilly, brittle crust and the largely solid mantle is the ball-shaped core.

Earth was a homogeneous ball of hot rock when it first formed roughly 4.5 billion years ago. The ball heated up even more due to radioactive decay and heat left over after planetary formation (the collision, accretion, and compression of space rocks).

After roughly 500 million years, our young planet's temperature reached 1,538° Celsius (2,800° Fahrenheit), the temperature at which iron melts. The iron age refers to this crucial period in Earth's history.

Therefore, The Earth’s core has two different parts is true about the earth's layer.

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Which of the following helps determine past climate on the basis of tree rings?
O oxygen isotope analysis
O pollen analysis
O dendrochronology
O 18O/16O ratio
O all of the above

Answers

If the year dates of the tree growth rings can be identified, the scientific discipline of dendrochronology can indeed be utilised to establish when a tree was cut down or died naturally. Seasonal climate shifts affect a tree's annual growth, which varies throughout the year.

Dendrochronology's goal is what?

The study known as dendrochronology, sometimes known as tree-ring dating, uses the annual growth rings that trees create to determine dates on a calendar (Nash 2000). This precise and sophisticated dating technique has been employed by archaeologists for nearly a century.

What dendrochronology principles are there?

According to this theory, there must have been physical and biological mechanisms in place in the past that connect present environmental processes with the patterns of tree development that exist today. James Hutton first said this in 1785, and it is now often understood to mean "the current is the keys to the past."

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Which describes the physical weathering of rock by water?


A. Water breaks down a rock into smaller pieces with the same composition as the larger rock.
B. Water dissolves rock into a solution.
C. Water carries away pieces of rock.
D. Any break down of a rock is the same as physical weathering of rock by water.

Answers

Answer:

it should be C. If not, try D.

Explanation:

Physical weathering of rock by water happens when waters affects the shape of a rock in any sort of form, such as breaking it into small pieces, breaking off a large chunk or etc.

The statement describes the physical weathering of rock by water - A. Water breaks down a rock into smaller pieces with the same composition as the larger rock.

physical weatheringis the process that breaks rocks apart without changing their chemical composition.water can contribute to the physical weathering of the rocks by is one of the reasons that can lead to cracking of rock caused by the freezing and thawing of water.

Thus, The statement describes the physical weathering of rock by water - A. Water breaks down a rock into smaller pieces with the same composition as the larger rock.

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The peptidoglycan layer of Gram-negative bacteria is
sandwiched between two cell membranes.
TRUE or
FALSE

Answers

The peptidoglycan layer of Gram-negative bacteria is sandwiched between two cell membranes is True.

Briefing:

Through the hydrophobic head of lipoprotein molecules known as Braun's lipoproteins, the peptidoglycan layer is non-covalently attached to them. The periplasmic area contains a concentrated gel-like matrix called the periplasm, Sandwiched between the outer membrane and the plasma membrane.

What purpose does a peptidoglycan function?

Peptidoglycan, which is a structural component of the bacterial cell wall, balances the osmotic pressure in the cytoplasm and offers structural support. Additionally, during bacterial cell division, peptidoglycan takes part in binary fission.

What cells have peptidoglycan in cell walls?

Peptidoglycan is a component of the cell walls of both gram positive and gram negative bacteria (also known as murein). Other than in the bacterial cell walls, this specific chemical has never been discovered anywhere else on Earth.

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Help y'all


If we are to start with 1000 grams of Carbon 14, how much do you expect to have left after 1 half life? After a second half life? After a third half life?

Answers

After one half life, you would expect to have 500 grams of Carbon 14 left. This is because half life is the amount of time it takes for half of the original amount of a radioactive material to decay.

After a second half life, you would expect to have 250 grams of Carbon 14 left. This is because half of the remaining 500 grams of Carbon 14 has decayed. After a third half life, you would expect to have 125 grams of Carbon 14 left.

This is because half of the remaining 250 grams of Carbon 14 has decayed. This pattern of halving the amount of Carbon 14 after each half life continues until all of the Carbon 14 has decayed.

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the first part of cellular respiration is called glycolysis, takes place in the _ and does not require any_

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The first part of cellular respiration is glycolysis. It takes in the cytoplasm and does not require any oxygen.

What is glycolysis?

Glycolysis is the first stage of cellular respiration. In this reaction, glucose molecules are broken down to produce two pyruvate molecules - 3 carbon compounds.

The process of glycolysis takes place in the cytoplasm of cells and happens in a two steps reaction:

Glucose is first phosphorylated to form glucose-6-phosphateThe glucose-6-phosphate produced undergoes isomerization to form fructose-6-phosphateFructose-6-phosphate undergoes phosphorylation to become fructose-1, 6-biphosphatefructose-1, 6-biphosphate cleaves to produce two 3-carbon molecules known as pyruvates.

Thus, glycolysis requires two molecules of ATP for the two phosphorylation reactions. It is a process that does not require oxygen.

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the area of the retina on which the light rays focus is the

Answers

Answer:

Lens

Explanation:

The area of the retina on which the light rays focus is the fovea, which is a small, central pit located in the retina that is responsible for vision, and there are rod and cone cells present in the human eye too.

What is the significance of the eyes?

There are several types of eyes present in the ecosystem, such as eye patterns, and the composition of the human eye is different from that of the dog and the cow, but this is the organ of vision, which allows the individual to see the world for nutrition, predation, navigation, communication, etc. Apart from that, it provides visual information about the shape, size, and color of objects and is involved in many other functions, such as the sleep-wake cycle,

Hence, the area of the retina on which the light rays focus is the fovea, which is a small, central pit located in the retina that is responsible for vision, and there are rod and cone cells present in the human eye too.

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how would the cylindrical shape of the palisade mesophyll?

Answers

It is not typical for a palisade mesophyll to have a cylindrical shape, as it is a type of plant tissue found in leaves and is typically arranged in layers. The cylindrical shape is not a characteristic that is commonly associated with the palisade mesophyll.

what is a greenhouse gas that has a global potential (gwp) that is greater than 1 and its anthropogenic source.

Answers

The greenhouse gas that has a global potential (gwp) greater than 1 and is an anthropogenic source is carbon dioxide (CO2).

CO2 is produced by the burning of fossil fuels and other human activities such as deforestation and cement production. Its gwp value is 1 by definition, but it remains the largest contributor to human-caused climate change due to the enormous amount of CO2 emissions from human activities. Other greenhouse gases with a higher gwp include methane (CH4) and nitrous oxide (N2O), but their anthropogenic sources are relatively smaller than CO2.

The global warming potential, or GWP, is a measurement of how much a certain greenhouse gas contributes to global warming over a given time period. It is typically compared to the GWP of carbon dioxide over the same time period. The ability of the gas to absorb and reemit heat radiation in the atmosphere, or its radiative forcing, is used to determine the GWP values.

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True or False: Ocean currents exist because of prevailing winds​

Answers

Answer. False

Explanation:

They occur because of the moon

A student adds 10 drops of a copper sulfate and sodium hydroxide solution to a test tube containing chicken broth. The solution undergoes a color change from blue to purple.
The presence of which organic molecule is indicated by the color change of the solution? A.Responses B. nucleosome C. protein D. fatty acids and glycerol E. glycogen

Answers

Correct answer is C. The presence of protein molecule is indicated by the color change of the solution

A standard solution is a liquid that contains an element or chemical in a precisely determined concentration. The solute is dissolved in the designated volume at a known mass. It is prepared using a primary standard or another material that must comply to requirements.

Qualities of solutions that depend more on the proportion of solute to solvent particles than on the characteristics of the individual particles are known as coagulative properties.

The different concentration units can be related using the number ratio. One molal is another name for a solution that has a concentration of 1 mol/kg. Molar mass must be represented in kg/mol rather than the more widely used g/mol or kg/kmol when using the unit mol/kg.

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Answer: Correct answer is C. The presence of protein molecule is indicated by the color change of the solution

what action do researchers take to make advances in science?

Answers

They share their experimental results with other researchers

Answer:

They share their experimental results with other researchers.

Explanation:

if they don't share there experience result to each other than it would take time to research. example I. one experiment there would be more than 10 scientist who will research on same thing. so to make eas

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compare the approximate diameter of the superior vena cava with that of the aorta. which has thicker walls?. Why do you suppose these differences exist?

Answers

The approximate diameter of the superior vena cava is 3.7 cm, while that of the aorta is approximately 2.5 cm. The walls of the aorta are thicker than those of the superior vena cava because the aorta has to withstand greater pressure.

What is the superior vena cava?

The superior vena cava is a large vein that carries deoxygenated blood from the head, arms, and upper body to the heart's right atrium. The superior vena cava is one of two veins that bring blood to the heart; the other is the inferior vena cava. The superior vena cava is formed by the junction of the two brachiocephalic veins.

The aorta, on the other hand, is the body's largest artery. It transports oxygenated blood from the heart's left ventricle to the rest of the body. The aorta is a thick-walled, elastic artery with a diameter of about 2.5 cm. The walls of the aorta are thicker than those of the superior vena cava because the aorta has to withstand greater pressure.

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Three functions of the eye help!!!!!

Answers

Explanation:

The eye receives oxygen through the aqueous. Its function is to nourish the cornea, iris, and lens by carrying nutrients, it removes waste products excreted from the lens, and maintain intraocular pressure and thus maintains the shape of the eye. This gives the eye its shape. It must be clear to function properly.

Answer:

These layers lie flat against each other and form the eyeball.

The outer layer of the eyeball is a tough, white, opaque membrane called the sclera (the white of the eye). ...

The middle layer is the choroid. ...

The inner layer is the retina, which lines the back two-thirds of the eyeball.

What is the specific role of chlorophyll a and chlorophyll b in a leaf in simple terms?
(100 points)

Please don't copy-paste stuff online.

Answers

Answer:

Function of chlorophyll a is to release chemical energy.

Function of chlorophyll b collects the energy in order to pass it to chlorophyll a

Explanation:

what is organelle
a part
of a cell that has a special function, much like an organ.
a genus
of sphere-shaped or oval bacteria. They occur in chains and cause many
illnesses in humans, including tonsillitis and strep throat.

Answers

An organelle is option A- a part of a cell that has a special function, much like an organ in a multicellular organism.

Organelles are membrane-bound structures that are found within the cytoplasm of eukaryotic cells. Examples of organelles include the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, peroxisomes, and chloroplasts.

Each organelle performs a specific function that is essential for the overall functioning of the cell. The concept of organelles is one of the defining features of eukaryotic cells and distinguishes them from prokaryotic cells, which lack membrane-bound.

In addition to the organelles mentioned earlier, there are several other types of organelles found in eukaryotic cells. For example:

Ribosomes: These are small, non-membrane-bound structures that are involved in protein synthesis. Ribosomes can be found in the cytoplasm or attached to the endoplasmic reticulum.

Cytoskeleton: This is a network of protein fibers that provides structure and support to the cell. The cytoskeleton is composed of three types of fibers: microfilaments, intermediate filaments, and microtubules.

Vacuoles: These are membrane-bound sacs that store materials such as water, nutrients, and waste products. Plant cells have large central vacuoles that help maintain turgor pressure and support the structure of the plant.

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A garden has a length of 1.5 x102 m and a width of 0.5 x 102 m. what is the area of the garden?

Answers

Answer:

7.5 × 10^3 m^2

Explanation:

The garden described in this question takes the shape of a rectangle, which has a length of 1.5 x102 m and width/breadth of 0.5 x 102 m.

To calculate the area, we use the formula for calculating the area of a rectangle, which is;

Area = length (L) × width (w)

Area = 1.5 x102 m × 0.5 x 102 m

Area = 150 × 50

Area = 7500

This, the area of the garden is 7500m^2 or 7.5 × 10^3 m^2

a cells is to a tissue as an atom is to a
choices;
subatomic particle
molecule
electron
population

Answers

Answer:

MOLECULE

Cells constitute tissues and atoms constitute molecules.

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according to the phylogenetic tree in the accompanying figure, g. intestinalis constitutes a ________ group. 1.Paraphyletic, 2.Monophyletic, 3.Polyphyletic, 4.Divergent.

Answers

According to the phylogenetic tree in the figure above, G. intestinalis constitutes a Paraphyletic group. So option 1 is correct.

A paraphyletic group of organisms is made up of an ancestor and some of its offspring, but not all of them belong to the same.

A paraphyletic group, then, excludes one or more monophyletic groups that are related to it through a shared ancestor.

The term "Reptilia," which contains reptiles, snakes, turtles, and crocodiles but not birds, is an example outline of a paraphyletic bunch.

Reptilia should be considered a paraphyletic group since birds and the rest of the reptiles share a shared ancestor.

Complete Question:

According to the phylogenetic tree in the accompanying figure, g. intestinalis constitutes a ________ group. 1.Paraphyletic, 2.Monophyletic, 3.Polyphyletic, 4.Divergent.

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according to the phylogenetic tree in the accompanying figure, g. intestinalis constitutes a ________

why can\'t benzaldehyde (shown below) react with itself by the aldol dehydration reaction?

Answers

Benzaldehyde cannot undergo aldol dehydration reaction with itself because it lacks alpha hydrogen atoms, which are required for the aldol reaction to occur.

The aldol reaction involves the addition of an enolate to a carbonyl group, followed by dehydration to form an alpha, beta-unsaturated carbonyl compound. In benzaldehyde, there are no alpha hydrogen atoms adjacent to the carbonyl group, so the formation of an enolate is not possible. Therefore, benzaldehyde cannot undergo aldol dehydration reaction with itself.
Benzaldehyde and the aldol dehydration reaction. Benzaldehyde cannot react with itself by the aldol dehydration reaction because it lacks an alpha-hydrogen. Aldol reactions require a molecule with an alpha-hydrogen to deprotonate, which then forms an enolate ion. Since benzaldehyde does not have an alpha-hydrogen, it cannot undergo the necessary steps to participate in an aldol dehydration reaction.

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What type of spatial distribution do the wolves illustrate?

Answers

Answer:

Could you please attach an image of the document so I can better assist you?

Explanation:

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in human blood type there are four different phenotypes a, b, ab, and o. these phenotypes are controlled by three different alleles on one gene. what type of inheritance pattern does this phenotype exhibit

Answers

Co-dominance type of inheritance pattern is exhibited in the human blood type. The different phenotypes of human blood type are A, B, AB, and O.

What is Co-dominance?

Codominance is a pattern of inheritance, in which the two different alleles of a gene are expressed in an organism, and each allele makes a slightly different protein than normal. Both the alleles influence the genetic trait or are responsible for determining the characteristics of the genetic condition in a person. ABO blood group is an example of Codominance.

A trait that results from an allele which is independently and equally expressed along with the other alleles in an individual is a codominant trait. An example of codominant trait is a person of blood type AB, who have one allele for blood type A and another for blood type B.

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What microscope has magnification ability of up to 60,000 times without losing clarity? TEM SEM STM

Answers


A scanning electron microscope (SEM)

sutures have a little movement??

Answers

Sutures have limited mobility or slight movement within their range.

The borders of a wound or incision are frequently held together during medical operations using sutures, also known as stitches. Sutures do permit some movement, despite being designed to hold the tissues in place and encourage healing.

This slight movement is intentional and serves a few purposes. Firstly, it accommodates the natural movements of the body without placing excessive tension on the wound, which can hinder healing. Secondly, it allows for drainage of fluids and minimizes the risk of fluid accumulation.

Lastly, the slight movement of sutures assists in reducing scar formation by distributing tension along the incision line. However, it is important to note that excessive movement or tension on sutures can compromise wound healing and should be avoided.

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Which infinite geometric series diverges?A. S= 4.2 + 3.57 + 3.0345 +...B. S= 262 + 301.3 + 346.5 +...C. S= 6,651 + 729 -- 81 -- ...D. S = 100 + 50 + 25 +... (b) (2 points) Find the curl of F(x, y, z) = (x^y, yz?, zx2) (c) (2 points) Determine if F = r+ y ln x is conservative (d) (2 points) Find the divergence of F = (ez?, 2y +sin (z2z), 4z + V x2 +9y2 Which environmental agency found itself under investigation after it was discovered that it was making mutually beneficial agreements with environmental polluters?A. Environmental Protection AgencyB. GreenpeaceC. U.S. Department of AgricultureD. World Wildlife Fund Figure A is a scale image of Figure B. If a*b=(a-b), calculate 2*5 Below is the derivation of the dynamic model for a double pendulum system. First, what assumptions were necessary to arrive at the given equations of motion. Then, convert the given equations to state-space form. lava is a hot liquid rock. when lava flows over solid ground beneath it increases in temperature. what happens to the molecules in the solid ground when the temperature is increasing? How do you determine where to place the decimal point in the product( please quick i have 10 other q to answer) piper inc. is a large cosmetics company that made an initial small investment in a start-up company, oscorp, which was developing an organic face lotion. this gave piper controlling interests in the start-up company. however, oscorp soon began to have financial difficulties because of principal-agent problems. as a result, piper did not invest in the next stage of development and pulled out of the company. this approach to strategic alliance is referred to as a multiple choice partial joint venture. break-even analysis. credible commitment. real-options perspective. Water flows through a cylindrical pipe of radius 0.74 cm.It fills a 12 l bucket in 4 minutes. When the 12 l bucket is emptied in to a circular pool, the water level rises by 5 mm. Calculate the radius of the pool. What is 150 rounded to the nearest 10th divided by 4 1.00 g of a compound is combusted in oxygen and found to give 3.14g of CO2 and 1.29 g of H2O. From these data we can tell thatA. the compound contains C, H, and some other element of unknownidentity, so we cant calculate the empirical formula.B. the compound contains only C and H and has the empirical formulaof C6H.C. the compound contains C, H, and O and has the empirical formulaof CH3O.D. the compound contains only C and H and has the empirical formulaof CH2.E. None of the above is a true statement The quote, It just gives me a good feeling to see the park looking so nice, represents _____. A.a main idea that is being implied B.a stated main idea C.a detail not related to the main idea TRUE/FALSE. life can be studied on a hierarchy of levels. from the following options, please choose the answer with items arranged from largest to smallest. the first heart sound, s1, also known as lubb, corresponds to the ___________. Ann worked 14 hours and earned a total of $108.50.How much did she earn per hour? Which context clue provides the best hint for the meaning of the underlined idiom in this excerpt?But Lauras inventing days were overthe rolling suitcase everyone else in the family remembersYoyo thinks of the speech her mother wrote as her last inventionafter that, her mother had passed on to Yoyo her pencil and pad please help ill give brainliest Why is China called the Middle Kingdom?Group of answer choices1.China is surrounded by invaders on all sides.2.Chinese legend has it that it is surrounded by the sky above and the sea below.3.China is located in the middle of two oceans.4.China is surrounded by natural barriers on all sides. Tomas is playing a board game with a cube numbered 1 through 6.He has to roll a 5 and then another 5 to win.What is the probability of him winning on his next roll? 7th grade math PLEASE HELP OR IM GONNA DIE THANK U