please select all of the materials and procedures necessary to clone a gene into a bacterium. assume the gene to be cloned has been isolated and purified.
a.DNA gene to be cloned
b.Transformation
c. ddNTPs
d. Cloning vector
e.Restriction enconucleases
f.RNA polymerase
g. Restriction endonucleases
h.RNA polymerase
i.Screening for recombinants
j.Bacterium serving as host (cloning host)
Answers
The materials and procedures required to clone a gene into a bacterium are:a. DNA gene to be cloned b. Transformation c. ddNTPs d. Cloning vector e. Restriction endonucleases f. Bacterium serving as host (cloning host) g. Screening for recombinants Restriction endonucleases are proteins that are responsible for recognizing and cutting DNA sequences, usually at specific points of the gene's sequence.
The gene cloning process involves the isolation of a particular gene from the entire genome and insertion of the cloned gene into the bacterium using various materials and procedures. The materials and procedures required to clone a gene into a bacterium are:a. DNA gene to be cloned b. Transformationc. ddNTPsd. Cloning vectore. Restriction endonucleasesf. Bacterium serving as host (cloning host)g. Screening for recombinantsRestriction endonucleases are proteins that are responsible for recognizing and cutting DNA sequences, usually at specific points of the gene's sequence. Cloning vectors are used to transform the DNA gene into the bacteria's cells. The most common bacterial host used for cloning is E. coli, which has been extensively studied and has a well-understood genome.Transformation is the process by which foreign DNA is introduced into bacterial cells. It occurs naturally in many bacterial species, but can also be induced in the laboratory using a variety of methods. The selection of recombinant cells is performed by using suitable marker genes. The recombinant cells are identified by their resistance to the selective agent and absence of the non-recombinant cells. Therefore, these are the materials and procedures necessary to clone a gene into a bacterium.
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Related Questions
the product of the light reactions of photosynthesis that gets used during
the Calvin cycle is
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I don't know it can some one help me please
Why do cells age and die?
No one knows for sure
Radiation
Protein changes
Changes in molecular shape
Answers
The cell age and die because of changes in proteins. The correct option is C.
What are cells?
Cells are living units that together form an organism. They contain cell organelles, and the cell organelle performs the carious function of the body. Cells are made up of macromolecules, like protein, lipids, and carbs.
There are various types of cells that perform different functions of the body. Old cells die and new cells are made from the old cells. The old cells die because of a change in the protein structure of protein denaturation.
Thus, the correct option is C. Protein changes.
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When humans burn fossil fuels, ________________ is released back into the atmosphere.
Answers
Answer:
Carbon Dioxide
Explanation:
Answer:
Carbon Dioxide
which of the following is a parasitic relationship
Answers
Answer:
I think It is the barnacles
Answer:
its A
Explanation:
For each biomolecule below, match the benefit provided to the body
a. Protein 1. Insulation and long-term energy
b. Lipids 2. Build lean muscle and strengthen tendons
c. Carbohydrates 3. Repair and Replicate DNA and RNA
d. Nucleic Acids 4. Provides quick, easily digested energy
Answers
Answer:
1. Protein - build lean muscle and strengthen tendons
2. Lipids - insulation and long-term energy
3. Carbohydrates - provides quick, easily digested energy
4. Nucleic Acids - repair and replicate DNA and RNA
Explanation:
a. Think of protein as what helps your muscles grow and strengthen
b. Think of lipids as fats (blubber, the extra fat, helps insulate seals from the cold)
c. carbohydrates are found in starch and bread products, and are used to help build up energy prior to marathons.
d. nucleic acids - think of nucleus; it is where genetic material is located, and also where DNA and RNA is located.
The Florida panther was once considered to be a subspecies of cougars. Current scientists, however, have studied the Florida panther and removed the subspecies classification
Which of the following would explain why the classification of the Florida panther changed?
A.) New genetic evidence suggests a much closer relationship between the Florida panther and other cougars
B.) Advanced technologies have shown a large difference in cell structure between the Florida panther and other cougars.
C.) Study of their embryos shows major differences in the development of the Florida panther fetus when compared to other cougars
D.)Shared physiological features
between the Florida panther and other cougars were also found in newly discovered organisms in the world.
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Explain what could happen if your kidneys stopped working??
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If your kidneys stopped working toxins, waste and water would build up in your body. You might feel fatigued, the whites in your eyes may appear yellow, you could feel nauseous and urinate less frequently.
What is a segment of dna on a chromosome that codes for a specific trait?
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How does a stormwater runoff system store water? Stormwater that cannot be directed back into rivers or streams is stored in.
Answers
Answer:
The storm drainage system helps prevent floods by diverting rainwater into nearby waterways. During rainstorms, water runs off of buildings, roads and other hard surfaces whilst picking up trash and pollutants along the way.The storm drainage system carries rainwater directly to creeks, streams, ponds and reservoirs.
Storm water that cannot be directed back into rivers or streams is stored in retention ponds.
Explanation:
Basalt is a gray or black igneous rock. Pilar uses an absolute dating method to study a sample of basalt. What will the method help her learn about the basalt sample?
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Choose one development in the fields of biology and discuss its contributions to humans.
Answers
Answer:
One massive development in human biology involves the use of 3D printers and human stem cells. 3D printing is developing to such a level that it can print basic replacement parts for human beings.
One development in the field of biology is genetic engineering. In humans, this methodology is implemented in the production of medically important products like human insulin, human growth hormone, hepatitis B vaccine, etc.
What is Genetic engineering?Genetic engineering may be characterized as a type of methodology that significantly utilizes a series of laboratory-based technologies in order to change the DNA makeup of an organism including humans.
In this modern world of science and technology, genetic engineering plays a crucial role in the development of medicine that include the manufacturing of drugs, the creation of model animals that mimic human conditions and genes, etc.
This process is also used to design to help humans overcome many genetically undesired diseases or traits among organisms including humans as well.
Therefore, one development in the field of biology is genetic engineering.
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What animal that has a similarity of humans reproduction, and its difference.
Answers
Answer:
Chimpanzees has a similarity of humans reproduction, and its difference is explained below in details.
Explanation:
Researchers discovered back in 2006 that chimpanzees experience around 98.4 and 99 percent of our DNA. They're more like to people than they are to gorillas! There are several similarities between animals and humans that you may have remarked. Humans and animals both feed, relax, imagine, and interact. We are also related in a lot of the means our bodies work.
a. Plants use CO2 in the process of ___________________ to make___________ and oxygen. b. Animals use oxygen in the process of _______________ and make more CO2. c. The ____________________________ is the main regulator of CO2 in the atmosphere because CO2 dissolves easily in it. d. In the past, huge deposits of carbon were stored as dead plants and animals ______________________. e. Today these deposits are burned as fossil fuels, which include ____________________, _____________________, and ___________________________. f. More CO2 is released in the atmosphere today than in the past because of the _________________________ . g. Too much CO2 in the atmosphere may be responsible for the _________________________________ effect/global warming.
Answers
a. Plants use CO2 in the process of photosynthesis to make glucose (sugar) and oxygen.
b. Animals use oxygen in the process of cellular respiration and make more CO2.
c. The oceans are the main regulator of CO2 in the atmosphere because CO2 dissolves easily in them.
d. In the past, huge deposits of carbon were stored as dead plants and animals in the form of fossil fuels.
e. Today these deposits are burned as fossil fuels, which include coal, oil, and natural gas.
f. More CO2 is released into the atmosphere today than in the past because of human activities, such as burning fossil fuels and deforestation.
g. Too much CO2 in the atmosphere may be responsible for the greenhouse effect/global warming,
where increased concentrations of greenhouse gases trap heat and lead to rising temperatures on Earth.
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One difference between photosynthesis and cellular respiration is that cellular respiration:
Answers
The main difference between photosynthesis and cellular respiration is that photosynthesis is an anabolic process, where the synthesis of organic compounds occurs, storing energy whereas cellular respiration is a catabolic process, where the stored organic compounds are utilized, producing energy.
In the human brain, the occipital lobe, controls vision. Which of the letters on the diagram above indicated the occipital lobe of the human brain?
A) e
B) a
C) d
D) c
Answers
Answer:
A) e
Explanation:
Answer:
option. A
E. represent the optical lobe of the human brain
hope it helps
Structure that organizes motion of the chromosomes?
Answers
Answer:
Structure that organizes motion of chromosomes. Cytoplasm. Material in cell; contains chemical wealth: sugars, amino acids, and proteins a cell uses to carry out everyday activites. Vacuole. Saclike structure (large in animal cell); stores water, salts, carbs, and proteins. Plays a role in disposing waste.
Explanation:
There are various structures that are found present in a cell. And these structures have peculiar functions they carry out in the cell. Chromosomes are found in the nucleus of a cell. During cell division there is the needed movement of the chromosomes.
The structure that organizes motion of the chromosomes is the centriole
The centriole is a structure found in the animal cells near the nucleus that aid in the movement of chromosomes through the action of the microtubule and spindle fibres. They help to assemble these microtubules and spindle fibres to aid movement of chromosomes.Learn more: https://brainly.com/question/24552946
In vampires, the ability to go outside during the day is recessive, while going out only at night is dominant. A female “day-dwelling” vampire marries a male heterozygous “night-dweller.” On scratch paper, use a Punnett square to determine the possible genotypes of their offspring. What is the genotypic ratio of their offspring?
Answers
Answer:
it be half and half
Explanation:
because the child will take 50% for the parents
The 56 chromosomes a baby sea turtle got from its parents contain all of its genes. Each of those genes is composed of a segment
of what biological polymer?
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What is the reason for having eyebrows
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If a transmembrane domain of protein contains beta-sheet structure, the overall structure of the transmembrane portion is likely a________. a) beta-barrel. b) beta-tube. c) beta-turn. d) beta-helix.
Answers
If a transmembrane domain of protein contains beta-sheet structure, the overall structure of the transmembrane portion is likely a beta-barrel.
The transmembrane domain of membrane proteins is a crucial domain that crosses the hydrophobic phospholipid bilayer of the membrane. The transmembrane domain is hydrophobic because it's composed of nonpolar amino acid residues, which are essential for crossing the membrane.
There are two forms of transmembrane domains: alpha-helical transmembrane domains and beta-barrel transmembrane domains, depending on their structural features. The transmembrane domain of alpha-helical proteins is an alpha-helix that runs through the membrane.The transmembrane domains of beta-barrel proteins, on the other hand, are beta-sheet structures that create a barrel-like shape by interacting with one another.
Therefore, if a transmembrane domain of protein contains beta-sheet structure, the overall structure of the transmembrane portion is likely to be a beta-barrel.
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What type of bonds are present in H2O2?
A.
covalent bonds
B.
nonmetallic bonds
C.
metallic bonds
D.
ionic bonds
Answers
Hope that helped
PLSSS HELP ILL GIVE 100 POINTS PLSSS HELPPP
Include a Claim, Evidence, Explanation, and Conclusion for full credit.
Cells were obtained from a patient with a viral infection. The DNA extracted from these cells consisted of two forms: double-stranded human DNA and single-stranded viral DNA. The base composition of these two forms of DNA are shown in the image.
Construct an argument based on evidence to determine which form was the viral DNA and which form was the human DNA.
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Answer:
TGCA
Explanation:
GCSE Science - Biology
Question 7 of 40
What word describes how much of a drug should be given?
Enter your answer
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Answer:
A little not more
Explanation:
Because it damages the body
WHOEVER DOES THIS ILL GIVE 100 points
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Answer:
file:///C:/User/Invitado/Downloads/dfe79f3cecac4f6cfdee738597a29b53.pdf
Explanation:
Please help and hurry!!
Describe one of the four inventions for deep-sea exploration covered in the lesson, and explain the specific uses for which it is designed.
Answers
One invention for deep-sea exploration is the remotely operated vehicle (ROV), designed for scientific research, sample collection, and underwater surveying.
One of the inventions for deep-sea exploration covered in the lesson is the remotely operated vehicle (ROV). ROVs are robotic underwater vehicles designed to explore the depths of the ocean where humans cannot easily reach. They are equipped with cameras, lights, and a variety of scientific instruments. The specific uses of ROVs include conducting research, collecting samples, and surveying underwater ecosystems. ROVs can be used to study marine life, geology, hydrothermal vents, and archaeological sites. They play a crucial role in gathering data and imagery for scientific research, oil and gas exploration, and underwater construction and maintenance. With their maneuverability and real-time video feeds, ROVs allow scientists and engineers to observe and document the underwater environment, enabling a better understanding of the deep-sea world and its resources, as well as supporting various industries that operate in the oceanic realm.For more such questions on Deep-sea exploration:
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how are saturated and unsaturated fatty acids different
Answers
Answer:
They are distinct because saturated fatty acids have hydrocarbon chains connected by single bonds solely. Unsaturated fatty acids have one or more double bonds. Each double bond may be in a cis or trans configeration.
Explanation:
How did Hershey and Chase prove that DNA is the genetic material?
Answers
Bacteriophages (viruses that infect bacteria) were important to Hershey and Chase's experiment, which demonstrated that DNA is the genetic material.
Hershey and Chase demonstrated that when bacteriophages, which are made up of DNA and protein, infect bacteria, the DNA enters but the majority of the protein does not. Hershey and Chase, as well as other findings, all contributed to the conclusion that DNA is the hereditary material. The Hershey-Chase studies, undertaken in 1952 by Alfred Hershey and Martha Chase, helped to prove that DNA represents genetic material.
While biologists had known about DNA since 1869, many scientists still concluded that proteins contained the information for heredity at the time since DNA looked to be an inert molecule and, because it is situated in the nucleus, its purpose was thought to be phosphate storage.
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A grassland biome occupies much of the center of north america. why is this, in terms of environmental factors?
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Because there is enough rain to sustain aban dant grass but not enough to sustain trees in the heart of North America.
What effect does logging have on the taiga biome?Logging and the more harmful practise of clear cutting, which involves the removal of significant regions of all trees, are endangering the taiga ecosystem's health. Trees not only offer habitat for wildlife, but they help maintain soil integrity.
The taiga biome, like many other woods, is threatened by deforestation. Humans are chopping down trees by the hundreds, and the taiga is gradually vanishing. This obviously has a detrimental influence on the forest since many animals lose their habitats and are forced to relocate.
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looking under a microscope, you note that the cell is a prokaryote. how do you know?
it lacks the cell membrane
it lacks nucleus
it lacks genetic material
it lacks cytoplasm
Answers
Answer:
It lacks nucleus.
Explanation:
Prokaryotic: cells that lack a true nucleus or membrane bound organelles.
Pro- before Karyo- NucleusA type of grass is planted in someone's backyard to look visually appealing but has a toxin that prevents local animals from successfully eating it. This new grass becomes popular among homeowners and is planted all over the local area, becoming the most common type of grass found. What statement explains the most likely impact of this new grass on the biodiversity of the area
The grass has improved the habitat and increased biodiversity
The grass has caused adaptations within the ecosystem and caused and increase in biodiversity.
The grass has become dominant and increased biodiversity.
The grass has become dominant and decreased biodiversity