in malik et al. 2014, how was rho determined to be the target of inhibition by bicyclomycin

Answers

Answer 1

In the study conducted by Malik et al. in 2014, the researchers investigated the molecular mechanism of bicyclomycin, a specific inhibitor of bacterial transcription.

They discovered that bicyclomycin targets the transcription factor Rho, which is involved in the termination of RNA synthesis. To determine this, the researchers performed a series of experiments using in vitro transcription assays with different bacterial strains and mutants. They found that bicyclomycin inhibited the termination of RNA synthesis in the presence of Rho, but not in its absence.

Furthermore, the researchers showed that bicyclomycin directly binds to Rho and inhibits its ATPase activity, which is required for RNA termination. Therefore, it was concluded that Rho is the target of inhibition by bicyclomycin.


In Malik et al. 2014, Rho was determined to be the target of inhibition by Bicyclomycin through a series of experimental techniques. Researchers first identified Rho as a potential target due to its role in transcription termination. They then used in vitro transcription assays to demonstrate that Bicyclomycin could inhibit Rho-dependent transcription termination. Furthermore, they conducted experiments with purified Rho protein, showing that Bicyclomycin directly binds to Rho, leading to its inhibition.

Additionally, they observed a synergistic effect when Bicyclomycin was combined with other Rho inhibitors, supporting Rho as the primary target. Overall, these results confirmed that Bicyclomycin inhibits Rho in transcription termination processes.

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Related Questions

what is the term for propane and butane gases that can be liquefied under high pressure during the extraction of natural gas?

Answers

The term for propane and butane gases that can be liquefied under high pressure during the extraction of natural gas is liquefied petroleum gas (LPG).

Liquefied petroleum gas (LPG) refers to a mixture of propane and butane gases that can be compressed into a liquid state under high pressure. LPG is obtained as a byproduct during the extraction and processing of natural gas or crude oil.

The high-pressure compression of propane and butane gases allows them to be stored and transported in a liquid form, making them more convenient and efficient for various applications. LPG has a higher energy density compared to its gaseous form, making it a valuable source of fuel for heating, cooking, and industrial purposes. It is commonly used in residential, commercial, and industrial settings as an alternative to traditional fuels like coal or wood.

LPG is stored in specially designed tanks or cylinders and can be easily vaporized back into its gaseous form for use. The ability to liquefy propane and butane gases under high pressure enhances their storage, transportation, and versatility, making LPG a widely utilized and accessible energy source in many parts of the world.

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Which of these is a negative form of a plant tropism?
1.shoot gravitropism
2.phototropism
3.pea plant thigmotropism
4.root gravitropism

Answers

The negative form of a plant tropism is the shoot gravitropism. Therefore, the correct option is 1.

What is plant tropism?

Plant tropism is a biological phenomenon in which a plant organism grows or bends in response to a stimulus, such as light or gravity.

There are several types of plant tropisms, including:

Phototropism: This is the growth response of a plant to light.Gravitropism: This is the growth response of a plant to gravity.Hydrotropism:This is the growth response of a plant to moisture. Thigmotropism:This is the growth response of a plant to touch.

Shoot gravitropism is a negative form of plant tropism, as here the shoot is growing upwards against the pull of gravity. Hence, the correct option is 1.

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Capillaries in the heart keep blood flowing in one direction.
a. True
b. False

Answers

Answer:

A) True.

Explanation:

Capillaries then lead into small veins. Small veins lead to larger and larger veins as the blood approaches the heart. Valves in the veins keep blood flowing in the correct direction.

Hope it helps!

What is artificial selection??
A.) a natural process

B.) A process during which human intentionally breed organisms with desirable traits.

C.) A process during which humans speed up the process of reproduction in organisms.

D.) A process during which humans genetically modify an organisms DNA.​

Answers

artificial selection is a process during which humans intentionally breed organisms with desirable traits. The answer is B.

Answer:

i believe the answer is b

Explanation:

Hiiii! Good morning/afternoon/evening/night! I hope you all are doing wonderful and here are some fun memes :)

Hiiii! Good morning/afternoon/evening/night! I hope you all are doing wonderful and here are some fun
Hiiii! Good morning/afternoon/evening/night! I hope you all are doing wonderful and here are some fun
Hiiii! Good morning/afternoon/evening/night! I hope you all are doing wonderful and here are some fun
Hiiii! Good morning/afternoon/evening/night! I hope you all are doing wonderful and here are some fun
Hiiii! Good morning/afternoon/evening/night! I hope you all are doing wonderful and here are some fun

Answers

Thank you for the points! LOL, good day to you too! Thanks and brainliest?

Hii good morning!! Thank you for the points!!

some proteins and antibodies are susceptible to ph damage. in order to prevent this damage, what would be the most efficient and effective additional step to perform at the end of the elution process?

Answers

Some proteins and antibodies are susceptible to pH damage. In order to prevent this damage, the most efficient and effective additional step to perform at the end of the elution process would be to adjust the pH to the optimal range or to buffer the sample.

pH is a measure of acidity or alkalinity, with a scale of 0 to 14, with 7 being neutral. A pH of less than 7 is acidic, while a pH of greater than 7 is basic (or alkaline).It is important to buffer a sample because proteins and antibodies are susceptible to pH changes. A buffer solution is a solution that can resist changes in pH when small amounts of an acid or base are added to it. The pH at which a protein or antibody is most stable is known as the optimal pH. If the pH is below or above the optimal pH, the protein or antibody will be denatured. Therefore, it is critical to buffer the sample to prevent the pH from shifting outside the optimal range and to maintain the protein or antibody's structural and functional integrity.

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How does the deletion of the Baez affect the polypeptide chain formed during protein synthesis?

Answers

If a protein contains two or more different polypeptide chains, each chain is coded by a different gene.
Okay now I’m not 100% on this but I’m pretty sure I got the main idea I think

What is the species number of eubacteria?

Answers

4,000 species can be found around the world

Read the excerpt. Which element of an informational

text does the excerpt primarily show?

a. An effect of Dadaism

b. A thesis about Dadaism

c. An example of a Dadaist

d. A conclusion about Dadaists

Answers

An element of an informational text that the excerpt primarily show is C, an example of a Dadaist.

What is a Dadaist?

Dadaism is a literary and artistic movement based on deliberate irrationality and rejection of traditional artistic values. Dada was an art movement founded in Zurich during World War I in response to the horrors and folly of the war.

Dada artists' art, poetry, and performance are frequently satirical and nonsensical in nature. Marcel Duchamp submitted a urinal to the Society of Independent Artists in 1917. The Society refused Fountain because they believed it was not a work of art.

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The bloodstream carries glucose as well as

oxygen to cells. They are the reactants for

cellular respiration, which occurs in the

cytoplasm and mitochondria. The process

generates ATP, the energy-carrying

molecule of the cell, and the blood carries

away waste byproducts.

What byproduct of respiration do you

predict will diffuse into the bloodstream?

Answers

The byproduct of cellular respiration that I predict will diffuse into the bloodstream is carbon dioxide

Cellular respiration is a metabolic process that occurs in the cytoplasm and mitochondria of cells, converting glucose and oxygen into ATP (adenosine triphosphate), the primary energy-carrying molecule in cells. During this process, glucose is broken down, and oxygen is used to produce ATP, water, and carbon dioxide.

Carbon dioxide is a waste byproduct of cellular respiration, and it needs to be removed from the cells to maintain a proper cellular environment, it diffuses into the bloodstream, where it is transported to the lungs. Once in the lungs, carbon dioxide is expelled from the body through the process of exhalation. The bloodstream plays a crucial role in transporting essential nutrients like glucose and oxygen to cells, as well as removing waste products such as carbon dioxide, ensuring the efficient functioning of our body's cells. So therefore carbon dioxide is the byproduct of cellular respiration that I predict will diffuse into the bloodstream.

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Why is North Carolina the most ecologically unique state in the southeast?

Answers

Answer: North Carolina is the most ecologically unique state in the southeast because its borders contain sub-tropical, temperate, and boreal habitats.

Explanation: Although the state is at temperate latitudes, the

Appalachian mountains and the Gulf Stream influence climate and, hence, the vegetation (flora) and

animals (fauna).

the formation of endodermal and ectodermal germ layers occurs at ________.

Answers

The formation of endodermal and ectodermal germ layers occurs at gastrulation.

Early in the development of the embryo, a crucial process called gastrulation takes place. This encompasses cell movement and reorganisation throughout the embryo, culminating in the ectoderm, mesoderm, and endoderm that constitute the three basic germ layers. Gastrulation is the process in early embryonic development where the blastula transforms into a three-layered structure called the gastrula.

The blastula, a hollow ball of cells, endures substantial changes throughout gastrulation. The cells penetrate and generate a feature known as the blastopore. This blastopore eventually gives rise to an incision known as the primitive streak by cell movement. The cells move and arrange themselves into the three germ layers at that point.

The ectoderm, which is the top layer, is where the nervous system, skin, and hair are formed. The intermediate layer, known as the mesoderm, gives rise to structures like muscles, bones, and elements of the circulatory system. The innermost layer, the endoderm, gives rise to organs like the liver, lungs, and digestive system.

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A train car is to train as a __ is to a polymer.
a. polymerization.
b. monomer
c. polypeptide.
d. protein.

Answers

Answer:

Answer is B monomer as multiple monomers makes up polymers

It would have to be B. Monomer

Which endocrine gland releases a hormone to regulate calcium levels in the bloodstream ? A. Thyroid B. Pituitary gland C. Adrenal gland D. Pancreas

Answers

Answer:

b

Explanation:

Parathyroid glands release a hormone to regulate calcium levels in the bloodstream the regulation of calcium levels in the blood.

Explanations:

Endocrine glands are the gland that secret hormone directly into the blood for creating certain stimuli. Parathyroid glands produce parathyroid hormone, which plays a key role in the regulation of calcium levels in the blood. The parathyroid glands are two pairs of small, oval-shaped glands. They are located next to the two thyroid gland lobes in the neck. Each gland is usually about the size of a pea.

functions:

Release of calcium by bones into the bloodstream

Absorption of calcium from food by the intestines

Conservation of calcium by the kidneys

Stimulates cells in the kidney to transform weaker forms of vitamin D into the form that is strongest at absorbing calcium from the intestines

       therefore, option A is the correct answer.

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Population Dynamics Lab Report ________________________________________ Instructions: As you complete each slide of the Population Dynamics Virtual Lab Activity, please fill in this lab report with the appropriate information and data. Title: Objective(s): Hypotheses: Variables: Materials: 1. The Population Dynamics Virtual Lab Activity 2. Population Dynamics Lab Report Procedures: The procedures are listed in Population Dynamics Virtual Lab Activity. You do not need to include them here. Data and Observations Table 1: Predation and Carrying Capacity Trial Starting Seal population Starting Whale population Highest Seal Population Highest Whale Population 1 25 25 2 25 3 25 Table 2: Growth Rate and Capture Efficiency Trial Seal birth rate Whale birth rate Highest Seal Population Highest Whale Population 1 0. 05 0. 005 2 0. 005 Table 3: Death Rate and Capture Efficiency Trial Whale death rate Whale capture efficiency Highest Seal Population Highest Whale Population 1 0. 05 0. 005 2 0. 005 Conclusion: Be sure to answer the following reflection questions as a summary in the conclusion of your lab report: • Were your hypotheses correct or incorrect? Why or why not? • Using Table 1, which of the three trials produced the highest population for both the whales and the seals? Why do you think this trial had the best outcome for the whale and seal populations? • Using Table 2 and 3, explain how the birth rates of the seals and the death rates of the whales affected the population for both whales and seals

Answers

The Population Dynamics Lab Report is a document that needs to be completed while completing the Population Dynamics Virtual Lab Activity. The lab report should contain the appropriate information and data that is collected during the virtual lab activity.

Title: Population Dynamics Lab Report
Objective(s): To understand the factors that affect the population dynamics of seals and whales.
Hypotheses: The population of seals and whales will be affected by factors such as predation, carrying capacity, growth rate, capture efficiency, and death rate.
Variables: Independent variables include predation, carrying capacity, growth rate, capture efficiency, and death rate. Dependent variables include the population of seals and whales.

Materials:
1. The Population Dynamics Virtual Lab Activity
2. Population Dynamics Lab Report

Procedures: The procedures for the virtual lab activity are listed in the Population Dynamics Virtual Lab Activity.

Data and Observations:

Table 1: Predation and Carrying Capacity Trial

Starting Seal population Starting Whale population Highest Seal Population Highest Whale Population
1 25 25
2 25
3 25

Table 2: Growth Rate and Capture Efficiency Trial

Seal birth rate Whale birth rate Highest Seal Population Highest Whale Population
1 0.05 0.005
2 0.005

Table 3: Death Rate and Capture Efficiency Trial

Whale death rate Whale capture efficiency Highest Seal Population Highest Whale Population
1 0.05 0.005
2 0.005

Conclusion:
After completing the virtual lab activity, the following reflection questions should be answered as a summary in the conclusion of the lab report:

• Were your hypotheses correct or incorrect? Why or why not?
The hypotheses were correct in that the population of seals and whales were affected by factors such as predation, carrying capacity, growth rate, capture efficiency, and death rate.


• Using Table 1, which of the three trials produced the highest population for both the whales and the seals? Why do you think this trial had the best outcome for the whale and seal populations?
Trial 2 produced the highest population for both the whales and the seals. This trial had the best outcome because it had the right balance between predation and carrying capacity.

• Using Table 2 and 3, explain how the birth rates of the seals and the death rates of the whales affected the population for both whales and seals.
The birth rates of the seals and whales directly affected the population growth of both species. The higher the birth rate, the higher the population growth. The death rates of the whales also had a direct impact on the population growth of both species. The higher the death rate, the lower the population growth.

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Using the table, what is the correct mRNA sequence representing the addition of alanine and then
leucine to a protein?

Using the table, what is the correct mRNA sequence representing the addition of alanine and thenleucine

Answers

Answer: B GCUCUU

Explanation:

G goes with c and A goes with T but your replacing thymine with uracil

CGU CUU represent the correct mRNA sequence representing the addition of alanine and then leucine to a protein.

What are codons?

A codon is a sequence of three nucleotides (also known as a trinucleotide) that can be found in either DNA or RNA and acts as a unit of genomic information by either encoding a specific amino acid or indicating the end of the process of protein synthesis (stop signals). There are 64 distinct codons, which are: 61 of them are utilized to define amino acids, while 3 are the stop signals.

UAG, UAA, and UGA are the three different STOP codons that can be found in the genetic code. During translation, these codons indicate that the chain of polypeptides has reached its conclusion. Due to the fact that they do not code for an amino acid, these codons are also referred to as nonsense codons and termination codons.

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which four elements make up the bulk of living cells?

Answers

The four elements that make up the bulk of living cells are carbon, hydrogen, oxygen, and nitrogen.

All organic compounds, including the main biological molecules of cells including carbohydrates, lipids, proteins, and nucleic acids, are built on the basis of carbon.

The second most prevalent element in living cells is hydrogen, which forms bonds with other elements to produce a variety of biological compounds, including lipids and carbohydrates.

The process by which cells transform the energy held in glucose into a form that can be utilized by the cell, known as cellular respiration, requires oxygen, making it a vital component of life.

Nitrogen: Nitrogen plays a crucial role in the construction of numerous biological components, such as the amino acids that make up proteins and the nucleotides that make up DNA and RNA.

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identify the greenhouse gases that make the greenhouse effect possible. check all that apply.

Answers

Carbon dioxide, methane, nitrous oxide, hydrochlorofluorocarbons (HCFCs), hydrofluorocarbons (HFCs), and ozone in the lower atmosphere are the principal greenhouse gases whose concentrations are rising.

Human activities have increased the atmospheric carbon dioxide content by more than 50% since the start of the Industrial Revolution, from 280 ppm in 1750 to 421 ppm in 2022. (about 1750) in greenhouse gases. The last time the atmospheric carbon dioxide level was this high was over 3 million years ago. Despite the fact that the numerous natural carbon sinks in the carbon cycle already absorb more than half of the emissions, this rise has nonetheless taken place.

The complete question is:

Identify the greenhouse gases that make the greenhouse effect possible. check all that apply.

a) Carbon dioxide

b) methane

c) nitrous oxide

d) hydrochlorofluorocarbons (HCFCs)

e) hydrofluorocarbons (HFCs)

f) All

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diffusion is the movement of molecules from....

an area of high concentration to an area of low concentration

an area of low concentration to an area of high concentration

an area of equilibrium to an area of high concentration

Answers

Answer:

an area of high concentration to an area of low concentratioN

ONLY ONE ANSWER!!

Which is one effect that would most likely result from a tsunami?
death
drought
extinction
genetic change

Answers

Death - A tsunami involves an ocean which wouldn’t cause a drought because the mass of water extinction the tsunami as a whole wouldn’t destroy every living thing on earth but cause a large death toll genetic change wouldn’t occur

One of the consequences of tsunami happening in a place would be death and destruction of biodiversity.

What is a tsunami?

A tsunami is a natural disaster caused by multiple waves in water bodies that send large volumes of water inland.

The sudden displacement of water with a large force destroys lives and properties on land, especially the coastal lands.

Thus, one of the surest effects of tsunamis is death.

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Why is heating a first step in pcr amplification of extracted dna?

Answers

The heat breaks the hydrogen bonds of the original DNA sample and separates the DNA into single strands

Which question MOST likely was asked FIRST to lead to the development of recombinant DNA technology?

a) Can undifferentiated cells be used to repair parts of the body?

b) Can human genes be introduced into the cells of people with genetic disorders using a virus?

c) Can DNA be cut into fragments by restriction enzymes and then separate into unique patterns?

d) Can human genes be integrated into bacterial DNA so bacteria can copy the genes and produce their proteins?

Answers

A. (the question needs to be 20 characters long lol).

Please answer the following questions. ✨WHEN I CAN I WILL MARK BRILLIANT✨


_____ is the study of the relationships and interaction of living things with their environment.


An ______ consist of all the living and nonliving things that interactwith one another within a given area.


A _____ is made up of all the living organisms in an ecosystem.

Answers

Answer:

1)Ecology

2)Environment

3)Ecosystem

Explanation:

2. To know more about preservatives.
To know about the different methods of preservation.
a) Collect the wrappers of frozen and canned food items and milk.
b) Find about the preservatives present in these food items.
c) List the various preservatives and methods of their preservation.

Answers

A chemical preservative stops items from decomposing. Some preservatives are used to cure wood or metal, while others are applied to food. Food preservation is "the science that deals with the process of preventing food from decaying or spoiling so that it can be stored in a fit condition for future use." Food quality, edibility, and nutritional content are all guaranteed via preservation.

They are not tainted with harmful substances or dangerous organisms.It does not lose its excellent color, texture, taste, or nutritional content.

What are the different methods of Food Preservation?

Drying: The oldest method of food preservation is drying. By using this technique, water activity is reduced, preventing bacterial growth. Drying makes food lighter so it can be transported more readily. Modern drying methods include bed dryers, fluidized bed dryers, freeze drying, shelf dryers, spray drying, commercial food dehydrators, and household ovens, in addition to the traditional methods of using the sun and wind. Examples of dried items using this technique include meat and fruits, apples, apricots, and grapes.

Freezing: Food prepared and frozen is kept in cold storage. Although potatoes can be controlled in dark areas, potato preparations must be frozen.

Smoking: Food is cooked, flavored, and preserved through smoking, which exposes it to wood smoke. Meats and fish are typically smoked because the smoke has antibacterial and antioxidant properties. Assorted smoking techniques are employed, including hot smoking, cold smoking, smoke roasting, and smoke baking. Smoking increases the risk of cancer when used as a preservative.

Vacuum packing creates: By making bottles and bags impermeable, vacuum packing creates a vacuum. Bacteria perish because there is no oxygen in the newly generated vacuum. Typically applied to dry fruit.

Salting and Pickling: Curing, commonly referred to as salting, removes moisture from goods like meat. Pickling is the process of preserving food in a salt solution (brine) or vinegar (acetic acid); in Asia, food is sometimes preserved in oil. At a 20% concentration, salt kills and stops the growth of microorganisms. Pickling can be done in many ways, including chemical pickling and fermentation pickling. To extend the shelf life of commercial pickles, sodium benzoate or EDTA is added.

Sugar: Fruits can be preserved with sugar in syrup form or crystallized form, depending on whether the material to be preserved is boiled in the sugar until it crystallizes, like candied peel and ginger. Another use is for fruit superficially coated in sugar syrup and then glazed. Alcohol and sugar are combined with preserving upscale foods like fruit in brandy.

Lye: Lye, also referred to as sodium hydroxide, makes food alkaline and inhibits the growth of microorganisms.

Canning and bottling: Sealing cooked food in sterile bottles and cans is the definition of canning and bottling. Boiling the container destroys or weakens bacteria. Various amounts of time or space are used for cooking food. The food is more at risk of rotting after the can or bottle has been opened.

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PLS HELP IT’S DUE TODAY

PLS HELP ITS DUE TODAY

Answers

Genes linked on the same chromosome tend to be inherited together, violating the principle of independent assortment.

What is the effect of crossing over?

The relative position of genes is determined by calculating the frequency of crossing-overs between genes located on the same chromosome. The closer two genes are located to each other on a chromosome, the lower is the probability that the independent distribution in the next generation violates the principle of the opposite chromosomes.

Crossing-over between them is highly likely, resulting in the formation of recombinant chromosomes. The phenotype resulting from a genotype is determined by the same distribution.

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Which plant response is affected by photoperiod?; Which of the following is the factor that initiates flowering in long day plants?; Is directional growth responses of plants to a unidirectional source of light?; How do plants respond to a decrease in environmental temperature?

Answers

The flowering, setting of winter buds, and vegetative growth of plant response are affected by photoperiod.

The timing and length of day and night trigger a biological reaction known as photoperiodism. It regulates the development of winter buds, vegetative growth, and flowering. Detection of seasonal variations is vital to plant life.

The majority of plants are sensitive to photoperiod in many different ways, including seed germination, leaf formation rate, leaf blade length and width expansion, dry matter generation, and partitioning, in addition to its effects on generative growth.

The reaction to variations in day length that enables plants to adjust to seasonal changes in their environment is called photoperiodism.

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why are some types of organisms much more commonly fossilized than others?

Answers

Answer:

The enamel and dentin of teeth are harder than bone and as a result, teeth are more likely to be fossilized than other parts of a vertebrate.

Explanation:

I really need help my grades go in tonight and this is my last day you will get full points

I really need help my grades go in tonight and this is my last day you will get full points

Answers

Answer:

the flat horizontal lines indicate phase change

Explanation:

As the substance changes phase from solid to liquid and liquid to gas, the temperature remains contant due to latent heat energy.

Energy is being used to break the intermolecular bonds during phase change, therefore there is no change in temperature.

does lemon juice have more or less H+ ions than it has OH- ions? explain

Answers

Acids are substances that provide hydrogen ions (H+) and lower pH, whereas bases provide hydroxide ions (OH–) and raise pH. The stronger the acid, the more readily it donates H+. For example, hydrochloric acid and lemon juice are very acidic and readily give up H+ when added to water. ( source : https://courses.lumenlearning.com/wmopen-nmbiology1/chapter/the-ph-scale/ )

The phase of mitosis in which chromosomes line up on the equator of the spindle in the middle of the cell is

Answers

The phase of mitosis in which chromosomes line up on the equator of the spindle in the middle of the cell is metaphase.

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