glycolysis begins with a(n) ________ stage(s).

Answers

Answer 1
Glycolysis begins with an energy investment stage.
Answer 2

Glycolysis is the first step in cellular respiration, which is a process that extracts energy from glucose.

It is present in the cytoplasm of the cell and begins with the breakdown of glucose (a six-carbon molecule) into two three-carbon molecules of pyruvate. This process requires an investment of two ATP molecules. There are 10 reactions in glycolysis, which can be divided into two stages, namely the energy investment phase (reactions 1-5) and the energy payoff phase (reactions 6-10).Answer: Glycolysis begins with a one stage.  Explain:Glycolysis begins with a six-carbon glucose molecule breaking down into two three-carbon pyruvate molecules.

This process of glycolysis is a ten-step process that occurs in the cytoplasm of the cell. These steps can be divided into two stages: the energy investment phase and the energy payoff phase.In the energy investment phase, the cell has to invest two ATP molecules to activate glucose and ensure its split into two molecules of glyceraldehyde 3-phosphate (G3P). The first stage of glycolysis consists of reactions 1-5 and is the energy investment phase. In the second stage, reactions 6-10, the energy payoff phase, the cell recovers its investment by producing four ATP molecules, yielding a net gain of two ATP molecules.

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Related Questions

Yang is an agriscientist seeking to protect crops against pests. He selects plants that have shown a resistance to these pests. What MOST likely
will Yang do with these plants?
OA
breed them
OB
harvest them
OC. germinate them
OD
fertilize them

Answers

D fertilize them because it’s common sense

explain how hybrid breakdown maintains seperate species even if fertilization occurs

Answers

When two different species interbreed and produce hybrid offspring that are less fit or have reduced fertility, hybrid breakdown helps to maintain separate species even if fertilization occurs between them

Hybrid breakdown is a biological phenomenon that occurs .When two different species interbreed, their genetic material can mix and create new combinations of genes that may not be compatible with each other. In the first generation of hybrids, these genetic incompatibilities may not be immediately apparent, and the hybrids may be healthy and fertile. However, in subsequent generations, genetic incompatibilities may accumulate and lead to reduced fitness or sterility.Reduced fitness or sterility in hybrids is a result of genetic incompatibilities that cause problems during development, reproduction, or survival. For example, a hybrid may have difficulty in finding a mate of the same species, or its offspring may have reduced viability or fertility. As a result, hybrid offspring are less likely to successfully reproduce and pass on their genes to the next generation, thus preventing gene flow between the two species. The phenomenon of hybrid breakdown therefore serves as a mechanism that helps to maintain separate species by limiting the gene flow between them. Even if hybridization occurs, the resulting hybrids may have reduced fitness or sterility, which reduces their chances of producing viable offspring and contributing to the gene pool of either parental species. This helps to maintain genetic and reproductive isolation between species, allowing them to continue evolving separately and forming distinct genetic lineages.

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Hybrid breakdown is a post-zygotic reproductive barrier that can occur when two different species interbreed and produce hybrid offspring. It involves the breakdown or weakening of hybrid offspring in subsequent generations, which ultimately leads to the separation of the two species.

In the first generation, the hybrid offspring may be healthy and viable, but in later generations, problems may arise. In hybrid breakdown, the hybrid offspring of the first generation are fertile, but their offspring (the second generation) are either infertile or exhibit reduced fitness. This can be due to the expression of recessive genes that were previously hidden in the parental species or the accumulation of mutations in the hybrid genome. As a result, the hybrid population cannot produce viable offspring and therefore cannot interbreed with either parental species. This ensures that the two species remain separate and maintain their distinct genetic identities. In summary, hybrid breakdown is a mechanism that can maintain the separation of two species even if fertilization occurs. It acts as a post-zygotic barrier to prevent the hybrid offspring from producing viable offspring, which ultimately prevents the two species from merging into a single gene pool.

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16. How are convection currents in the asthenosphere associated with mid-ocean ridges?

Answers

Answer: GoodMorning: ) Ok so this happens When two oceanic plates are pushed away from each other, magma rises to the surface between the plates and creates a ridge.

Explanation: In  places where convection currents rise up towards the crust's surface, tectonic plates move away from each other in a process known as seafloor spreading (Fig. 7.21). Hot magma rises to the crust's surface, cracks develop in the ocean floor, and the magma pushes up and out to form mid-ocean ridges.

Have a Nice day <3 ✨

can someone help me?​

can someone help me?

Answers

The Rabbit and Mouse- Have a great day!

a man with a AB blood is married to a woman with AB blood. What blood types can their children have

Answers

Answer:

They will have a child with either any of the following A,B, OR AB

giardia intestinalis have a symmetrical heart shape morphology. True or False

Answers

The given statement, "Giardia intestinalis has a symmetrical heart-shaped morphology," is false because its morphology is characterized by an elongated or pear-shaped appearance, often resembling the shape of a "teardrop" or "pear."

Giardia intestinalis, commonly known as Giardia, does not have a symmetrical heart shape morphology. Giardia is a microscopic protozoan parasite that causes gastrointestinal infections in humans and animals. Its morphology is characterized by an elongated or pear-shaped appearance, often resembling the shape of a "teardrop" or "pear."

The organism has a distinct bilateral symmetry with two nuclei and flagella that allow it to move and attach to the lining of the small intestine. The heart-shaped morphology mentioned in the statement does not accurately represent the appearance of Giardia intestinalis.

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neuronal circuits, morphology-filling markers, and dopaminergic projections in the brain clearing tissue

Answers

Neuronal circuits are the connections formed between neurons in the brain that allow for the transmission of electrical signals and the processing of information. These circuits play a crucial role in various brain functions such as perception, memory, and movement.

Morphology-filling markers are substances or techniques used to visualize and map the shape and structure of neurons in the brain.

They can help researchers understand the intricate details of neuronal circuits by labeling and highlighting specific neurons or neural pathways.

Dopaminergic projections refer to the pathways in the brain that utilize the neurotransmitter dopamine. Dopamine is involved in various important functions such as reward, motivation, and movement.

These projections play a significant role in regulating mood, motivation, and coordination.

Brain clearing tissue is a technique used to make brain tissue transparent, allowing for a clearer view of the neuronal circuits and other structures within the brain.

This technique involves treating the brain tissue with chemicals or using specialized imaging methods to remove lipids and other substances that make the tissue opaque.

In conclusion, neuronal circuits, morphology-filling markers, and dopaminergic projections are all important components in studying the structure and function of the brain.

Neuronal circuits form the basis for information processing in the brain, while morphology-filling markers and brain clearing techniques help researchers visualize and understand the intricate details of these circuits.

Dopaminergic projections, on the other hand, play a crucial role in regulating important brain functions such as reward and movement.

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What is ONE major difference in the problems associated with obtaining measurements on the sizes or densities of animal populations versus plant populations?

Answers

I think one major difference with that is that animals can move around, go distances in search for food or mate, and thus make the animal densities per geography vary greatly. Birds migrate regularly, so their population densities tend to vary with seasons. Their mobility also depends on the availability of food, so animals go away if there are no food in the area.

Plants on the other hand don't move around faster (they can migrate by reproduction: it's their seeds moving around). Thus their densities tend to be more constant per season/life cycle.

A scientist wants to compare two cells: one from a liver and one from a heart. Which laboratory tool would be most helpful? and why?​

Answers

The most helpful laboratory tool is a microscope. Cells can not be seen with the naked eye, so we must use a telescope to compare the cells.

Do racial categories change and why?

Answers

Answer:

The OMB states, "many federal programs are put into effect based on the race data obtained from the decennial census (i.e., promoting equal employment opportunities; assessing racial disparities in health and environmental risks). Race data are also critical for the basic research behind many policy decisions.The most recent United States Census officially recognized five racial categories (White or European American, Black or African American, American Indian, Alaska Native, Asian American, Native Hawaiian, and Other Pacific Islander) as well as people of two or more races.

Explanation:

brainliest?

What are the three types of ecological pyramids? Explain how each type of pyramid
models energy and matter in ecosystems.

Answers

Answer:  The three types of ecological pyramids include the pyramid of number, which shows the number of individual organisms that exist at different trophic levels; the pyramid of biomass, which tell us the amount of living tissue or the total mass of organisms within each trophic level; and the pyramid of energy, which indicates the amount of energy at each trophic level and the actual role of each organisms in the process of energy transfer.

The three types of the ecological pyramid are-

the pyramid of numberthe pyramid of biomassthe pyramid of energy

Ecological pyramids show the different amounts of energy or matter present within each trophic level in a particular food chain or food web. The three different types are Pyramids of energy, biomass, and numbers.

The pyramid of number - represents the number of individual organisms that exist at various trophic levels;The pyramid of biomass- the amount of living tissue or the total mass of organisms within each trophic levelThe pyramid of energy - a graphical representation of the energy found within the trophic levels of an ecosystem.

Thus, the correct answer is - Pyramids of energy, biomass, and numbers.

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What are the three types of ecological pyramids? Explain how each type of pyramidmodels energy and matter

Critical thinking : Do magnets need to touch a piece of iron or another magnet to exert a magnetic force on the iron or other magnet?

Answers

Answer:

I wouldn't think so because it is the magnetic field that Attracts and repels. When iron is magnetised to something don't the molecules lineup in the North the South pattern or something like that to create a magnetic field and stick together.

I might be wrong because this is my personal opinion on the matter.

Answer:

no because the magnetic force attracts the magnet or the iron to it

Explanation:

Imagine that you wanted to measure a hormone using a reaction where increasing amounts of the hormone caused the reaction solution in a test tube to turn a darker color. What procedures would you use to determine the precise concentration of the hormone in an unknown sample?

Answers

To determine the precise concentration of the hormone in an unknown sample, you would use a procedure called a colorimetric assay. This involves the following steps:

1. Prepare a series of standard solutions with known concentrations of the hormone.
2. Perform the reaction on each standard solution and the unknown sample, resulting in a color change.
3. Measure the absorbance of light at a specific wavelength for each standard solution and the unknown sample using a spectrophotometer.
4. Create a calibration curve by plotting the absorbance values against the known concentrations of the hormone in the standard solutions.
5. Determine the concentration of the hormone in the unknown sample by finding the corresponding absorbance value on the calibration curve.

By following these steps, you can accurately measure the hormone concentration in your unknown sample using the colorimetric assay.

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describe the characteristics of a person with disabled mirror neurons

Answers

Mirror neurons are a type of brain cells that are believed to play a role in understanding and imitating the actions, emotions, and intentions of others. While mirror neurons are present in most individuals, some individuals may have impaired or disabled mirror neurons, which can affect their ability to empathize, imitate, and understand the actions and emotions of others.

A person with disabled mirror neurons may exhibit the following characteristics:

Difficulty with empathy: Mirror neurons are thought to contribute to empathy, the ability to understand and share the feelings of others. Individuals with disabled mirror neurons may struggle to empathize with others, finding it challenging to understand and relate to the emotions and experiences of those around them.

Limited imitation skills: Mirror neurons are involved in imitation, allowing individuals to observe and replicate the actions of others. A person with disabled mirror neurons may have difficulty imitating others, finding it challenging to learn through observation and mimicry.

Impaired social interaction: Mirror neurons are implicated in social cognition and understanding social cues. A person with disabled mirror neurons may struggle with social interactions, finding it challenging to interpret and respond appropriately to nonverbal cues, gestures, and facial expressions.

Reduced ability to learn through observation: Mirror neurons play a role in observational learning, where individuals learn by watching others. Those with disabled mirror neurons may have difficulty learning through observation, relying more on explicit instruction or trial-and-error methods.

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After a signal binds to a signal receptor, the next step is transduction. Why is the signal transduction step necessary?.

Answers

The correct option is (C).

The more molecules that share the responsibility of passing the signal to activate the effector the more quickly and efficiently the message can spread.

What is Signal transduction?

The process by which a cell responds to substances outside the cell through signaling molecules found on the surface of and inside the cell.Three steps can be identified in cell signaling: A cell recognizes a signaling chemical coming from outside the cell, Transduction: The receptor protein is altered in some way when the signaling molecule attaches to it and finally, the signal causes a particular biological reaction.Because multicellular animals must coordinate the actions of hundreds to trillions of cells, signal transduction systems are particularly crucial in these systems. Cell-to-cell communication is made possible by a variety of methods that multicellular organisms have created.

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I understand that the quesion you are looking for is "After a signal binds to a signal receptor, the next step is transduction. Why is the signal transduction step necessary?

(A) The signal transduction pathway is not necessary since the signal can directly activate the effector.

(B) Transmission of the signal through multiple relay molecules before activating an effector is not efficient.

(C) The more molecules that share the responsibility of passing the signal to activate the effector the more quickly and efficiently the message can spread.

(D) The efficiency increases when less molecules share the responsibility of moving the signal from one molecule to the next to activate the effector."

a) what is the primary difference between the von neumann architecture and the architecture employed by the arm cortex m?

Answers

The primary difference between the Von Neumann architecture and the architecture employed by the ARM Cortex M is the way in which they handle memory and data processing.

The Von Neumann architecture is based on the concept of a single, shared memory space that is used for both data storage and instruction execution. This means that data and instructions are stored in the same memory and accessed through the same bus. This can lead to a bottleneck in performance, as the CPU has to wait for data and instructions to be fetched from memory before it can begin processing.

In contrast, the ARM Cortex M architecture employs a Harvard architecture, which separates data and instruction memory into two distinct spaces. This allows for faster processing, as the CPU can fetch data and instructions simultaneously from separate memory spaces.

Additionally, the ARM Cortex M architecture includes a number of specialized registers and instructions that are optimized for embedded and real-time applications.

Overall, the primary difference between the Von Neumann architecture and the ARM Cortex M architecture is the way in which they handle memory and data processing, with the ARM Cortex M architecture offering faster performance and specialized features for embedded and real-time applications.

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the result of an infection by a microbe called a superantigen can cause an excessive, uncontrolled release of cytokines called a(n) which can lead to a dangerous decrease in blood pressure, shock, multi-organ failure, and potentially, death.

Answers

Superantigen type toxins lead to the release of excessive amounts of cytokines stimulating a hyperactive immune response that can lead to shock and death in the affected individual.

In the field of biology, such a kind of antigen that is responsible for the production of an excessive immune response is referred to as a superantigen.

As a result of superantigen-type toxins invading the body, more cytokines will be produced. This is because cytokines are sites of the body that are involved in the production of immune cells.

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Complete each sentence by dragging the proper label into the appropriate position.
Word in () is the answer

Answers

The sentence that complete the proper label into the appropriate position are 1) digestive process 2) Chemical monomers 3) Indigestible residues  5) mechanical and chemical breakdown of food 4) Fecal matter

1. The digestive process begins with the (ingestion) of foods.

2. Starting in the mouth, the mechanical and chemical breakdown of food is referred to as the process of (digestion).

3. Once foods are broken down into chemical monomers, the digestive system works to move nutrients from the GI tract into the cells of the body via (absorption).

4. Indigestible residues along with some of the non-reabsorbed secretions of digestive organs, undergo (compaction) where the fluids are removed and a remaining solid is created.

5. The final step requires (defecation), where the fecal matter resulting from compaction is excreted from the body

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Energy transformations result in
a.) a loss of energy
b.) a gain of energy
c.) a loss or gain in energy depending on the situation
d.) no loss or gain in energy

Answers

Answer:

c

Explanation:

depends on what you are transforming it into

Simple diffusion requires energy to move materials through the membrane that is why it is called passive.a. Trueb. False

Answers

False (b). Diffusion is a kind of process that does not require energy.

Microorganisms, plants, and animals all benefit from the diffusion of tiny molecules as well as macromolecules (like proteins) in aqueous solutions. Diffusion plays a significant role in the drying of liquid mixes and solutions as well as the preparation of food. For example, during evaporations, scent components in fruit juice, coffee, and tea diffuse from solutions.

Products, waste, and occasionally enzymes diffuse out during fermentation while nutrients, oxygen, and sugar diffuse to the microbes. Urea, creatinine, and extra fluid are all removed from the blood by the kidneys. Patients with dysfunctional kidneys receive renal dialysis to eliminate waste materials from their blood.

The hemodialysis procedure involves pumping the patient's blood through a dialyzer while waste diffuses to the aqueous cleaning fluid across a semipermeable membrane.

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Which of the following best describes a benefit of non-renewable energy use?

A) Scientific advances now allow us to synthesize fossil fuels making them renewable

B) Ecosystems will be healthier as dangerous chemicals in fossil fuels are extracted for use as energy

C) Removing these energy sources will stabilize the abiotic factors in the ecosystem making them more resilient to change

D) The infrastructure to distribute this energy is already in place, therefore less surrounding environments need to be disturbed​

Answers

Answer:

No d is the right answer

Explanation:

the infrastructure to distribute this energy is already in place ,therefore less surrounding environment need to be disturbed.

it is important for an embryo initially to have a large supply of rrna because ____

Answers

An embryo initially needs a large supply of rRNA because of its role in protein synthesis.

Why is a substantial amount of rRNA important for an embryo?

During the early stages of development, an embryo undergoes rapid growth and cellular division. These processes require a significant amount of protein synthesis to build the necessary structures and carry out essential functions. Ribosomal RNA (rRNA) plays a crucial role in protein synthesis as it forms the core component of ribosomes, the cellular machinery responsible for assembling proteins.

Ribosomes consist of two subunits, one large and one small, which come together during translation to facilitate the synthesis of proteins. The large subunit contains rRNA molecules that help catalyze the formation of peptide bonds between amino acids, ultimately leading to the creation of proteins. Therefore, a large supply of rRNA is vital for an embryo to efficiently produce proteins and support its rapid growth and development.

The initial abundance of rRNA in an embryo ensures that there are sufficient ribosomes available to meet the high demand for protein synthesis. As development progresses, the embryo's cells will differentiate into specialized tissues and organs, each with specific protein requirements. The ample supply of rRNA allows for the synthesis of diverse proteins necessary for the proper formation and functioning of these specialized structures.

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What will happen if the pollen tube pore its content inside the embryo sac?

Answers

Answer:

Once the embryo sac has developed, pollination has occurred, and the pollen tube has grown into the ovary to make contact with the ovule, fertilization (fusion of egg and sperm) can occur.Typically, the pollen tube reaches the embryo sac via the micropyle (Greek, mikros + pyle = small opening), or opening, in the integuments of the ovule.. There, it discharges its sperm into the embry

Explanation:

The pollen tube enters into the ovule, through the micropyle. Inside the embryo sac, the tip of the pollen tube ruptures and the 2 male gametes are set free near the egg apparatus. Inside the embryo sac, one of the 2 male gametes fuses with the egg nucleus and forms a diploid zygote. This process is called syngamy or true fertilisation.

Answer:

In angiosperms, the pollen tube releases

its contents ( including sperm cells) in

to the embryo sac upon insertion into the

ovule, thus completing double

fertilization

13. How did Kettlewell test his hypothesis?
HELP I HAVE TO TURN THIS IN NEXT PERIOD

Answers

Kettlewell conducted an in-depth investigation of bird predation on moths to verify his theory regarding the evolutionary basis of industrial melanism.

When he carried out this investigation, Kettlewell tested what exactly?

Kettlewell came to the conclusion that industrial melanism, which darkened the hue of the trees, was brought on by pollution from the companies in Birmingham. As a result, the moths with the recessive features had a higher likelihood of surviving due to the concealment.

The goal of Kettlewell's experiment was to determine whether a single instance of natural selection could be explained in terms of a particular process or agent, in this case, bird predation. Biologists would not have abandoned the theory of natural selection if it had failed. Most biology textbooks include Kettlewell's experiment as an illustration of how evolution works.

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Which of the following statements about homologous chromosomes is true? They are genetically identical. In a cell with four pairs of homologous chromosomes - two pairs from each parent Homologous chromosomes can be found in both haploid and diploid mother cells They contain all of the same genes but not necessarily the same alleles Homologous chromosomes pair up and undergo recombination during prophase of mitosis How many dyads are found at the end of meiotic telophase I at each pole of the cell in an organism with a haploid number of 12? 48 24 18 12 6 Humans are diploid and have two copies each of 23 chromosomes. How many chromosomes would be present in a cell during prophase I of meiosis? 23 46 69 92

Answers

1. They contain all of the same genes but not necessarily the same alleles.

2. 24 dyads are found at the end of meiotic telophase I at each pole of the cell.

3. 46 chromosomes would be present in a cell during prophase I of meiosis.

1. Homologous chromosomes are similar but not identical. They carry the same genes in the same loci but may have different alleles, which are alternative forms of a gene. This genetic variation between homologous chromosomes contributes to genetic diversity.

2. During meiotic telophase I, the cell undergoes cytokinesis, resulting in two daughter cells. Each daughter cell will contain half the number of chromosomes found in the original cell. Since the organism in question has a haploid number of 12, there would be 24 dyads (pairs of sister chromatids) at each pole of the cell.

3. In humans, the diploid number of chromosomes is 46, meaning there are 23 pairs of homologous chromosomes. During prophase I of meiosis, homologous chromosomes pair up, resulting in a total of 46 chromosomes (23 pairs) present in the cell. This pairing and recombination during prophase I facilitate the exchange of genetic material between homologous chromosomes.

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6. Snyder ends her talk by


saying, "Darwin knew what


we seem to have forgotten,


that science is not only for


scientists. " What do you


think that is meant by this?


Do you consider yourself to


have a basic scientific


literacy? Explain and


discuss your thoughts.

Answers

Snyder's speech emphasize the significance of owning our own media literacy and using it to make informed decisions. Being media literate is an ongoing process, and we must constantly work on developing and refining our abilities.

In her talk, Snyder ends by saying, "We are the ones we've been waiting for." This statement implies that the responsibility of promoting media literacy and critical thinking lies with each individual person. It means that we should not wait for others to provide us with the necessary tools to navigate the complex media landscape. Instead, we should take an active role in developing our own media literacy skills and using them to make informed decisions.

Regarding the question of whether I consider myself to be media literate, I would say that I am continually working to improve my skills. With the abundance of information available today, it is essential to develop the ability to evaluate sources critically, identify biases, and recognize misinformation. I believe that being media literate is an ongoing process, and it requires a willingness to engage in lifelong learning.

In conclusion, Snyder's statement emphasizes the importance of taking responsibility for our own media literacy and using our skills to make informed decisions. Being media literate is an ongoing process, and it is essential to continuously work on developing and refining our skills.

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rigor mortis usually sets in about nine hours after death occurs.true or false

Answers

Rigor mortis usually sets in about nine hours after death occurs. It takes roughly 12 hours for rigor mortis to become fully established and about another 12 hours for it to start fading away as the body begins to decompose.

Correct answer is : True

Rigor mortis usually sets in about two to six hours after death occurs. It takes roughly 12 hours for rigor mortis to become fully established and about another 12 hours for it to start fading away as the body begins to decompose. Rigor mortis, or body stiffening, is the result of a decrease in ATP after death.

ATP is required for the muscles to relax, and without it, they become rigid. Rigor mortis can last up to 48 hours, after which the body becomes increasingly pliable as the muscles decompose.

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PLEASEEE HELP!! Ill mark brainlist

How/why does the DNA separate?
Discuss porosity or pores, electricity, DNAS charge & size of DNA pieces. Highlight all the 4 terms

Answers

Electrophoresis is a technique commonly used in the lab to separate charged molecules, like DNA, according to size.

Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size.

Charged molecules move through a gel when an electric current is passed across it.

An electric current is applied across the gel so that one end of the gel has a positive charge and the other end has a negative charge.

The movement of charged molecules is called migration. Molecules migrate towards the opposite charge. A molecule with a negative charge will therefore be pulled towards the positive end (opposites attract!).

The gel consists of a permeable matrix, a bit like a sieve, through which molecules can travel when an electric current is passed across it.

Smaller molecules migrate through the gel more quickly and therefore travel further than larger fragments that migrate more slowly and therefore will travel a shorter distance. As a result the molecules are separated by size.

Gel electrophoresis and DNA

Electrophoresis enables you to distinguish DNA fragments of different lengths.

DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode.

Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.

The use of dyes, fluorescent? tags or radioactive? labels enables the DNA on the gel to be seen after they have been separated. They will appear as bands on the gel.

A DNA marker with fragments of known lengths is usually run through the gel at the same time as the samples.

By comparing the bands of the DNA samples with those from the DNA marker, you can work out the approximate length of the DNA fragments in the samples.

How is gel electrophoresis carried out?

Preparing the gel

Agarose gels? are typically used to visualise fragments of DNA. The concentration of agarose used to make the gel depends on the size of the DNA fragments you are working with.

The higher the agarose concentration, the denser the matrix and vice versa. Smaller fragments of DNA are separated on higher concentrations of agarose whilst larger molecules require a lower concentration of agarose.

To make a gel, agarose powder is mixed with an electrophoresis buffer and heated to a high temperature until all of the agarose powder has melted.

The molten gel is then poured into a gel casting tray and a “comb” is placed at one end to make wells for the sample to be pipetted into.

Once the gel has cooled and solidified (it will now be opaque rather than clear) the comb is removed.

Many people now use pre-made gels.

The gel is then placed into an electrophoresis tank and electrophoresis buffer is poured into the tank until the surface of the gel is covered. The buffer conducts the electric current. The type of buffer used depends on the approximate size of the DNA fragments in the sample.

Preparing the DNA for electrophoresis

A dye is added to the sample of DNA prior to electrophoresis to increase the viscosity of the sample which will prevent it from floating out of the wells and so that the migration of the sample through the gel can be seen.

A DNA marker (also known as a size standard or a DNA ladder) is loaded into the first well of the gel. The fragments in the marker are of a known length so can be used to help approximate the size of the fragments in the samples.

The prepared DNA samples are then pipetted into the remaining wells of the gel.

When this is done the lid is placed on the electrophoresis tank making sure that the orientation of the gel and positive and negative electrodes is correct (we want the DNA to migrate across the gel to the positive end).

Separating the fragments

The electrical current is then turned on so that the negatively charged DNA moves through the gel towards the positive side of the gel.

Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run.

The distance the DNA has migrated in the gel can be judged visually by monitoring the migration of the loading buffer dye.

The electrical current is left on long enough to ensure that the DNA fragments move far enough across the gel to separate them, but not so long that they run off the end of the gel.

Illustration of DNA electrophoresis equipment used to separate DNA fragments by size. A gel sits within a tank of buffer. The DNA samples are placed in wells at one end of the gel and an electrical current passed across the gel. The negatively-charged DNA moves towards the postive electrode. Image credit: Genome Research Limited

tank.

state the total number of deaths from TB in African​

Answers

Answer:

I think they reach over 500 milion

A person is lying on the bed gazing at the ceiling. She is the ____ position
A. Prone
B. Supine
C. Anatomical
D. Caudal

Answers

Answer:

Supine.

Explanation:

A person is lying on the bed gazing at the ceiling, meaning that she is laying on her back. This is the supine position, in which the face and front of body (torso) is facing the same direction, which is upwards.

When flipped, the body would be in the prone position.


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Other Questions
What branch may pass laws regarding issues not directly addressed in the Constitution About 25 percent of all children have at least one episode of sleepwalking, also known as somnambulism. Sleepwalking typically occurs during the first three hours after the child has gone to sleep. The child gets out of bed and moves about in a slow, poorly coordinated, automatic manner, usually with a blank staring look on his face. Surprisingly, the sleepwalking child is usually able to navigate around objects without much difficulty. However, the childs general lack of awareness of his surroundings is evident. The sleepwalker may try to dress, eat, or go to the bathroom in the wrong location. (myers and dewall, psychology) what is the primary pattern of organization in this passage? comparison and contrast definition cause and effect argument Why is Farquhar about to be executed on OwlCreek Bridge?O He was a Confederate soldier who was captured by aFederal scoutOSP He ran a plantation and was from an establishedslave-owning family.O He attempted to tamper with the Union-controlledrailroad bridge to aid the Confederate cause.OseHe spied on the Union by hosting a Federal scout. What does the gerund phrase "volunteering at the animal shelter contribute to the text?The phrase conveys significant interest and variety.The phrase adds a specific detail about time and place.The phrase acts as a noun that is the subject of the sentence.The phrase adds more details by providing a direct object. principle of insurable interest states that a person must be subject to emotional or financial hardship resulting from damage, loss, or destruction to obtain insurance. In a coordinate system in which the direction to the right is positive, what are the distance and displacement of a person who walks 35 m to the left, 18 m to the right, and then 26 m to the left? ANSWER FAST PLEASE!!!You and your friend would like to build a tent that is 6 feet wide by 4 feet tall by 8 feet long. You bought a piece of canvas that is 14 feet by 10 feet to be able to make the tent. Remember you will need to put canvas on the floor of your tent. Will you have enough canvas to build the tent? of the travelers arriving at a small airport, 60% fly on major airlines, 30% fly on privately owned planes, and the remainder fly on commercially owned planes not belonging to a major airline. of those traveling on major airlines, 50% are traveling for business reasons, whereas 60% of those arriving on private planes and 90% of those arriving on other commercially owned planes are traveling for business reasons. suppose that we randomly select one person arriving at this airport. what is the probability that the person you have ten blankets that each take up 686 cubic inches of space. how many blankets could you pack into an 18-inch moving box?A. 10 blanketsB. 9 blanketsC. 8 blanketsD. 7 blankets Assume that the demand curve D(p) given below is the market demand for widgets:Q = D(p) = 1308 - 13p, p > 0Let the market supply of widgets be given by:QS(p)=3+6p, p>0where p is the price and Q is the quantity. The functions D(p) and S(p) give the number of widgets demanded and supplied at a given price.What is the equilibrium price? A neutral atom has a mass number of 80 and has 45 neutrons check the periodic table and write its complete symbolic representation of this element.Another atom has two more protons and two more electrons as the atom above. Using the periodic table what is the complete symbolic representation of this element. TIntroduction to permutations and combinationsSuppose we want to choose 3 letters, without replacement, from the 4 letters A, B, C, and D.(a) How many ways can this be done, if the order of the choices is relevant?0(b) How many ways can this be done, if the order of the choices is not relevant?0X List One differences between dwarf planets and the others planets of our solar system. Astronomy. An entrepreneur invests in his dream business of selling golf-carts. The initial investment is $230,064.00. The entrepreneur expects to generate an annual after-tax cash flow of $55,966.00 in running this business. How long before the project will pay back List the time of year (June Solstice, December Solstice, Equinox) that the following locations will be receiving maximum insolation1. Mission Viejo2. Quito, Ecuador3. Nome, Alaska4. Sydney, Australia5. Nairobi, Kenya6. Buenos Aires, Argentina If a nation has a higher level of technology than another nation it means that they will be able to produce: Which of the following best characterizes the debate regarding the Second Amendment according to the author of the passage?AThe Second Amendment debate underscores the broader constitutional mission to balance the individual rights of citizens and the need for rule of law.BThe Second Amendment debate has been clouded by powerful interest groups who are influential at the federal level.CThe rights guaranteed by the Second Amendment, like those guaranteed by the First Amendment, require practical limitations, but striking the appropriate level of regulation has historically been a challenge.DThe Second Amendment is viewed by some as an individual rights issue but by others as a states' rights issue. A komodo dragon can grow to be 120 inches long. One Komodo dragon is 92 inches long A. Write an equation to find the number of inches it still needs to grow to be 120 inches long B. Solve the equation. How many inches does the Komodo dragon still need to grow to be 120 inches long? Which former slave took up the fight to gain freedom for the slaves? Anna is composing a song on her piano using c scale. What will most likely be true about her song?.