Place the steps of a PCR \& restriction-enzyme cloning workfiow into the correct order by dragging and dropping the text boxes to the correct position.
Answers
The steps of a PCR and restriction-enzyme cloning workflow are given below:1. PCR amplification of the DNA fragment with primers containing the desired restriction sites.2. Digestion of the PCR product and the cloning vector with the same restriction enzyme.3. Ligation of the digested PCR product and cloning vector.4. Transformation of the ligation mixture into a competent E. coli cell.5. Selection of the transformed E. coli cells on a selective medium containing the appropriate antibiotic.
The PCR is used to amplify the target DNA region by specific primers that contain the restriction sites for the enzyme that will be used in the next step. In the next step, the PCR product and cloning vector are digested with the same restriction enzyme and then ligated.
The ligation mixture is transformed into a competent E. coli cell, where the transformed cells are then selected using an appropriate antibiotic in a selective medium.
Therefore, the correct order of the steps in a PCR and restriction-enzyme cloning workflow are PCR amplification of the DNA fragment with primers containing the desired restriction sites, Digestion of the PCR product and the cloning vector with the same restriction enzyme, Ligation of the digested PCR product and cloning vector, Transformation of the ligation mixture into a competent E. coli cell and Selection of the transformed E. coli cells on a selective medium containing the appropriate antibiotic.
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Related Questions
The results of one experiment carried out by a research team would be considered valid if
A) All the members of the research team came to the same conclusion
B) The experiment had more than 1 variable
C) The experiment was repeated and same results were obtained each time
D) The experiment had no control set up
Answers
Answer:
c
Explanation: hope it helped
Which of the following reflects changes in Homo erectus from Homo habilis?smaller teeth and bigger brow ridgesbigger teeth and smaller brow ridgesthinner cranial bonea rapid decrease in body size
Answers
The primary change that reflects the transition from Homo habilis to Homo erectus is smaller teeth and bigger brow ridges.
Homo erectus evolved around 1.9 million years ago and displayed several distinct physical characteristics compared to its predecessor, Homo habilis.
Homo erectus had smaller teeth due to dietary changes, including the consumption of more meat, which required less chewing force. Additionally, they used tools for food processing, which lessened the need for large, powerful teeth.
Bigger brow ridges were another significant feature in Homo erectus, serving as a reinforcement for the skull structure. This development suggests an adaptation to the increased physical demands of their lifestyle, such as hunting and the use of more complex tools.
Though not as prominent, thinner cranial bones were also observed in Homo erectus, which can be attributed to a more efficient brain structure. This characteristic is consistent with their increased cognitive abilities, such as problem-solving and social behavior.
A rapid decrease in body size was not observed during this evolutionary transition; instead, Homo erectus exhibited a more robust body structure, increased height, and an overall increase in body size compared to Homo habilis. These features allowed Homo erectus to better adapt to various environments and improve their chances of survival.
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The following reflects changes in Homo erectus from Homo habilis is a. smaller teeth and bigger brow ridges
The transition from Homo habilis to Homo erectus is marked by several significant changes. Among the options provided, the one that best reflects this change is smaller teeth and bigger brow ridges. As Homo erectus evolved from Homo habilis, they developed smaller teeth, which is an indicator of a shift in their diet to include more meat and less plant material, this dietary change was likely a result of their increased use of tools and hunting techniques
Additionally, Homo erectus is characterized by more prominent brow ridges compared to Homo habilis, which is a feature related to the overall shape of their skull. The other options - bigger teeth and smaller brow ridges, thinner cranial bone, and a rapid decrease in body size - do not accurately represent the evolutionary changes between Homo habilis and Homo erectus. In fact, Homo erectus had a more robust and larger body size than Homo habilis, which enabled them to adapt to different environments and expand their range across the globe. So therefore the correct answer is a. smaller teeth and bigger brow ridges, is reflects changes in Homo erectus from Homo habilis.
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Name one activity that humans do that causes weathering
Answers
Answer:
burning coal
Explanation:
hop this is helpful
please help answer this quickly
Answers
Answer:
wow l like question... l love fish thank u
Answer:
Your answer is D
Hope this helps!
Which organelle is used in making molecules of glucose in autotrophs?
A. nucleus
B. Golgi body
C. chloroplast
D. mitochondria
Answers
A. nucleus
B. Golgi body
C. chloroplast
D. mitochondria
It’s C chloroplast
colorblindness is an x-linked recessive genetic trait. the frequency of the mutant colorblind allele is 1/50. what is the probability that k will be colorblind if he is male? it is not known if anyone in the family is colorblind
Answers
The probability of K being colorblind if he is male is equal to the probability of the mother carrying the mutant colorblind allele, which is 1/50.
Colorblindness is an X-linked recessive genetic trait, meaning it is associated with genes located on the X chromosome. In males, who have one X chromosome, the presence of the colorblind allele on that chromosome determines whether they will be colorblind or not.
The given information states that the frequency of the mutant colorblind allele in the population is 1/50, which means that in a random individual, there is a 1/50 chance of having the colorblind allele.
Since it is not known if anyone in K's family is colorblind, we assume that the mother's allele frequency is the same as the general population. Therefore, the probability of the mother carrying the mutant colorblind allele is also 1/50.
As K is male, he inherits his X chromosome from his mother. If his mother carries the mutant colorblind allele and passes it on to him, he will be colorblind. Thus, the probability of K being colorblind if he is male is equal to the probability of the mother carrying the mutant colorblind allele, which is 1/50.
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What type of questions can ice cores answer?
A. What the climate was like on earth in the past
B. Why the climate changed in the past
C.What type of wild-life lived on earth in those times
Answers
what is the role of proteins in transmitting pain messages?
Answers
hope this helps!!:)
Which would be an autotroph in a grassland ecosystem?
A. monarch butterfly
B. prairie cordgrass
C. mallard duck
D. muskrat
Answers
Answer:
B. Prairie Cordgrass
Explanation:
100% on the quick check
Autotroph organisms are capable of producing organic compounds from inorganic sources, such as light. All plants are autotroph organisms. The correct option is B. prairie cordgrass.
Let us review the differences between autotroph and heterotroph organisms.
What is an autotroph organism?
Organisms that can take inorganic substances, such as light, and turn it into food according to their own needs are producers, and they are called autotrophic organisms.
These organisms are by excellence all plants, that photosynthesize.
Autotrophs aborb 1% of the energy that reaches the earth's surface. This small percentage of energy flows through all organisms in the ecosystem -from autotrophs to heterotrophs- until it dissipates in the environment.
What is an heterotrophic organism?
Organisms that are incapable of producing their food are called heterotrophic.
They depend on other organisms from the trophic chain such as plants or other animals to feed on, so they can get proteins and energy.
In the trophic chain, heterotrophic organisms occupy different levels after producers.
The prairie cordgrass is the heterotrophic organism.
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dna technology is especially useful in detecting and treating human diseases that are
Answers
DNA technology is especially useful in detecting and treating human diseases that are genetic in nature.
What is DNA technology?
DNA technology is a set of techniques used to study and manipulate DNA, the genetic material found in all living organisms. DNA technology has enabled the detection and treatment of human diseases that are genetic in nature.
How is DNA technology useful in detecting human diseases?
Genetic diseases can be caused by changes in DNA sequences or mutations in a single gene. DNA technology can detect these mutations through genetic testing and gene sequencing.
For instance, diseases like cystic fibrosis and sickle cell anemia can be detected using DNA technology.
How is DNA technology useful in treating human diseases?
DNA technology can also be used to treat genetic diseases. One way this is done is through gene therapy. Gene therapy is a process of replacing, modifying, or supplementing the non-functioning gene with a functional one.
By using gene therapy, the root cause of the genetic disease is addressed rather than just treating the symptoms.
Summary, DNA technology is especially useful in detecting and treating human diseases that are genetic in nature. DNA technology is used to detect mutations in genes that cause genetic diseases. Gene therapy is a technique used to treat genetic diseases by replacing, modifying, or supplementing non-functioning genes with functional ones.
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Based on the weather map, what weather conditions would you predict for the Florida area? Use data to support your answer.
Answers
Answer:
high winds with heavy rain
Explanation:
based on the key their is a hurricane coming to Florida from the east with winds towards Florida in several cities
Based on the weather map, the weather conditions for the Florida area is high winds with heavy rain.
What is the weather?A mechanical process is physical weathering. The temperature changes within a rock typically have a significant impact on this. The outside of a rock warms up more quickly than the inside when it is heated by the sun.
As a result, the rock experiences stress internally and deferential expansion. The interior also cools more slowly than the exterior during cooling.
The rocks eventually shatter and fragment into smaller fragments after numerous chilling and heating cycles. When water freezes and thaws in rock fissures, the same mechanical process is at work. But throughout the process, the rock's chemical makeup stays the same.
Based on the key, a hurricane is headed towards Florida from the east, with gusts reaching many cities in Florida.
Therefore, according to the weather map, Florida is currently experiencing high winds and heavy rain.
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Gene on a chromosome lies close to another gene controlling a different trait. This indicates that jeans are blank the first gene contributes to the expression of the second gene. This indicates blank
Answers
Answer: genes are linked
Explanation: Gene on a chromosome lies close to another gene controlling a different trait. This indicates that genes are linked, meaning they are physically close to each other on the same chromosome. The proximity of these genes suggests that they are more likely to be inherited together as a unit, rather than independently assorting during the process of genetic recombination.
spent two more hours on the Interstate than on the highway. He averaged 75 mph on the Interstate and 65 mph on the highway. How many hours did he spend driving on the Interstate
Answers
He spent 8 hours driving on the Interstate.
To solve this problem, let's first define two variables: let x be the number of hours spent on the highway, and y be the number of hours spent on the Interstate. We know that he spent two more hours on the Interstate than on the highway, so we can express this relationship as y = x + 2. We also know his average speeds: 75 mph on the Interstate and 65 mph on the highway.
Next, we'll use the formula distance = speed × time. We know that the total distance driven on the highway and the Interstate is the same, so we can set up the equation: 65x = 75y. Now, we can substitute the expression for y from the first equation into the second equation: 65x = 75(x + 2).
Solve for x: 65x = 75x + 150, which simplifies to 10x = 150. Dividing both sides by 10, we find x = 15. Now, we can find y: y = x + 2 = 15 + 2 = 17. Therefore, he spent 17 hours driving in total, with 8 hours on the Interstate and 7 hours on the highway.
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What occurs with the help of transport proteins.
Answers
Answer: Transport proteins act as doors to the cell, helping certain molecules pass back and forth across the plasma membrane, which surrounds every living cell. In passive transport molecules move from an area of high concentration to an area of low concentration.
Which of these pumps oxygen poor blood to the lungs? a. Right ventricle. b. Left ventricle. c. Pulmonary artery.
Answers
The pump that pumps oxygenated blood to the lungs is the right ventricle. The answer is option (a) right ventricle.
A ventricle is one of the four chambers of the heart that pumps blood to different parts of the body. There are two ventricles, the right and the left ventricles. They are located at the bottom of the heart.
The left ventricle is larger and thicker than the right ventricle. It receives oxygenated blood from the lungs and pumps it out to the body through the aorta.
On the other hand, the right ventricle receives deoxygenated blood from the body and pumps it to the lungs through the pulmonary artery. It is thinner than the left ventricle because it only has to pump blood to the lungs, which is a shorter distance.
The right ventricle's main function is to pump blood to the lungs so that it can be oxygenated before it flows back to the left side of the heart for distribution throughout the body.
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Which is a tough and rigid layer that surrounds plant cells, as well as some algal and bacterial cells?
cell wall
centriole
nucleus
cell membrane
Answers
The tough and rigid layer that surrounds plant cells, as well as some algal and bacterial cells, is called the cell wall. Therefore, the correct statement is option A.
What are the functions of cell wall?The cell wall provides mechanical support to the cell, thereby, protecting it from damage caused by external factors. The cell wall consists of complex carbohydrates, such as cellulose, lignin, etc.
The cell membrane is selectively permeable, and the cell wall is porous allowing the passage of some molecules. The cell wall also helps in cell communication and signaling.
The cell wall determines the shape of the cell and helps in the regulation of growth and development. The thickness and composition of the cell wall change with respect to different environmental conditions and helps in the survival and function of many types of cells.
Therefore, the tough and rigid layer that surrounds plant cells is called the cell wall.
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¿el significado de comedia?
Answers
Answer:para hacer reír a la gente y hacer brillar su día
Explanation:
Indicate which form of hemopoiesis can improve the symptoms of each condition.Improved through erythropoesis:Sickle cell diseaseAnemiaHemolysisHypoxiaHemolytic disease of the newbornMalariaKwashiorkorHemorrhageTransfusion reactionsImproved through leukopoesis:LeukopeniaNOT improved through erythropoesis or leukopoesis:LeukemiaHematologyPrimary polycythemiaSecondary polycythemia
Answers
Currently, the only treatment available for some sickle cell disease patients is a bone marrow and blood transplant.
Your healthcare practitioner could provide medications or transfusions after an early diagnosis to treat problems, such as chronic discomfort. Eat well-balanced meals and drink glasses of water each day if you have sickle cell disease. Avoid becoming too hot, chilly, or exhausted. Children should engage in physical activity to maintain their health. The gene that instructs the body to produce hemoglobin, an iron-rich substance found in red blood cells, is altered in sickle cell anemia. Red blood cells deliver oxygen from the lungs throughout the body thanks to hemoglobin.
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I need the order please
Answers
What causes one embryo to develop unique characteristics compared to the embryos of different species?
A. The type of food available to the mother
B. The genes found in the chromosomes of the embryo
C. Environmental factors such as climate, air pollution, and water quality
D. The embryo begins as a smaller version of the fetus and simply increases in size
Answers
Answer:
Dont worry about me, I just added this answer so the other person can get their brainliest because they definetly deserve it Explanation:
Dont worry about me, I just added this answer so the other person can get their brainliest because they definetly deserve it Explanation:
The process of glycolysis produces 4 ATP total, but we say that the net ATP production is 2 ATP. What happened to the two other ATP molecules
Answers
In mature animals, cell division is mainly restricted to repairing tissues by __________ old or damaged cells.
Answers
Cell division in adult animals is mostly limited to repair and replacement. A cell develops many sub-cellular structures when it differentiates in order to perform certain functions. It now functions as a specialized cell. Chromosomes composed of DNA molecules are found in a cell's nucleus.
Our telomeres are long when we are born, but as we age and our cell division more frequently, they get shorter and shorter. The chromosomes are less protected when cell division telomeres shorten, and eventually the chromosomes are exposed to damage and cell division is halted.
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a multicolored calico cat was cloned, and it was found that each of the cloned offspring showed unique coloring patterns. which chromosome is the gene responsible for fur color in calico cats most likely to be on
Answers
If different cells randomly expressed a different copy of the X chromosome—one form of dosage compensation for the X chromosome—this could result in distinct coloring patterns in calico cats.
The question stem gives us a situation where a group of cats has the same genetic makeup but varied phenotypes. This must imply that variations in gene expression are to blame for the discrepancies in phenotypes. The X chromosome dosage compensation provides a method for this; in dosage compensation, females express just one copy of the X chromosome.
The Y chromosome is expressed in all cells in males. The text claims that there has been a large loss of Y-chromosome genes, while in truth, very few Y-chromosome genes are involved in expressing the masculine phenotype.
As a result, the X chromosome is the proper response. Calico cats may develop distinctive coloring patterns as a result of this.
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Your question is incomplete. Please find the complete question below.
A multicolored calico cat was cloned, and it was found that each of the cloned offspring showed unique coloring patterns. Which chromosome is the gene responsible for fur color in calico cats most likely to be on?
A. Y
B. X
C. An autosomal chromosome
D. Impossible to determine without more information
Fertilization occurs when _____.
Answers
Fertilization happens when a sperm cell successfully meets an egg cell in the fallopian tube.
EXPLANATION:
Once fertilization takes place, this newly fertilized cell is called a zygote. From here, the zygote will move down the fallopian tube and into the uterus. The zygote then burrows into the uterus lining.
How is subsistence use helpful to the ecosystem
Answers
Subsistence use can be helpful to the ecosystem when practiced in a sustainable manner. It relies on traditional ecological knowledge, helps maintain biodiversity, and promotes conservation practices.
Subsistence use often relies on traditional ecological knowledge, which is based on a deep understanding of the local ecosystem and its dynamics. This knowledge is often passed down from generation to generation, and it enables communities to use natural resources in a way that maintains the health and productivity of the ecosystem. Secondly, subsistence use can help maintain the biodiversity of an ecosystem. By relying on a variety of different natural resources, subsistence communities can help prevent the overexploitation of any one species or resource.
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The vessels that deliver oxygen to the tissues of the body are part of the __________.
Answers
Answer:
They are your blood vessels
Explanation:
Human anatomy
Gray wolves and killer whales share which of the following taxa?
O Delphinidae, Carnivora, Animalia
Animalia, Chordata, Mammalia
O Caidae, Cetacea, Mammalia
Animalia, Mammalia, Canidae
Answers
Answer:
B. Animalia, Chordata, Mammalia
Explanation:
Web
Cred not mine
Answer: The correct answer is Animalia, Chordata, Mammalia
Explanation: This answer has been confirmed correct.
Multiple choice please help, will give brainliest!
If a pedigree is Sex-linked that means
a.) only males can be carriers
b.) females can never get the disease
c.) females are always the carriers
d.) it is carried on the Y chromosome
Answers
Answer:
you don't answer me hello am I going to be mark brainliest this is the correct answer I just got done working on this and my son got it correct it is your answer is C females are always the carriers .
Explanation:
your explanation females cuz they have two X chromosomes which means they can be a carrier for a sex-linked trait . Please mark me brainliest
Punnet square Rr x Rr diagram
Answers
Answer:
RR,Rr,Rr,rr
Explanation:
Answer:
RR,Rr,Rr,rr
Explanation:
Just combine all the possible combinations.
Can our cells undergo photosynthesis? Why or why not?
Answers
Answer:
No, Human bodies cannot undergo photosynthesis.
Explanation:
Our body's demand for glucose is higher than photosynthesis can accommodate. We also don't have chloroplasts to be able to carry out photosynthesis.