Answer: (P2/P1)=(T2/T1)
(P2/1200.0kPa)=(387/283)
P2=(387K/283K) * (1200kPa)
P2= 1641kPa
Explanation:
An Oxygen Tank has a pressure of 1200 Kpa at 283K and when temp. is increased to 378 K then pressure will be 1,602.82 kpa.
How do we calculate the pressure?From the ideal gas equation it is clear that pressure of gas is directly proportional to the temperature, and for this question required equation will be:
P₁/T₁ = P₂/T₂, where
P₁ = initial pressure of oxygen tank = 1200 kpa
T₁ = initial temperature of oxygen tank = 283 K
P₂ = final pressure of oxygen tank = ?
T₂ = final temperature of oxygen tank = 378 K
On putting values, we get
P₂ = (1200)(378) / (283) = 1,602.82 kpa
Hence resultant pressure of the oxygen tank is 1,602.82 kpa.
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Air is cooling at night. The frost point (temperature at which RH with respect to ice reaches 100%) is reached at T = -10 degree Celsius. a) What is the RH (normal RH with respect to liquid water) at this point? b) Upon further cooling the air reaches a temperature of T =-11 degree Celsius Kaolinite particles of 200 nm diameter are present. Do you expect ice particles to form? If yes, do they form via deposition nucleation or condensation of droplets followed by freezing? Briefly explain your answer. c) Upon even further cooling the air reaches a temperature of T = -12 degree Celsius. Same question as before: Do you expect ice particles to form now? If yes, do they form via deposition nucleation or condensation of droplets followed by freezing? Briefly explain your answer. Equilibrium vapor pressures may be calculated or taken from the table below. t/°C 0 -1 -2 -3 -4 -5 -6 -7 -8 -9 - 10 -11 -12 -13 T/ Keow /Pa 273.15 611.2 272.15 568.2 271.15 527.9 270.15 490.2 269.15 454.8 268.15 421.8 267.15 390.9 266.15 362.1 265.15 335.1 264.15 310.0 263.15 286.5 262.15 264.7 261.15 244.3 260.15 225.4 259.15 207.8 258.15 191.4 e oi/Pa 611.2 562.7 517.7 476.1 437.5 401.8 368.7 338.2 310.0 283.9 259.9 237.7 217.3 198.5 181.2 165.3 - 14 - 15 Equilibrium vapor pressures with respect to water (eow) and with respect to ice (coi).
The equilibrium vapor pressure with respect to water (eow) is 259.9 Pa. assume that saturation vapor pressure is same as equilibrium vapor pressure.
Therefore, the RH at the frost point is
RH = (eow / saturation vapor pressure) × 100
= (259.9 Pa / 259.9 Pa) × 100
= 100%
b) At T = -11 °C, we need to compare the equilibrium vapor pressure with respect to water (eow) and the equilibrium vapor pressure with respect to ice (coi) to determine if ice particles will form. From the given table, at T = -11 °C, the equilibrium vapor pressure with respect to water (eow) is 237.7 Pa, and the equilibrium vapor pressure with respect to ice (coi) is 165.3 Pa.
The air is supersaturated with respect to ice, and the presence of Kaolinite particles can provide surfaces for water droplets to condense onto, leading to the formation of ice particles.
c) At T = -12 °C, we compare the equilibrium vapor pressure with respect to water (eow) and the equilibrium vapor pressure with respect to ice (coi). From the given table, at T = -12 °C, the equilibrium vapor pressure with respect to water (eow) is 217.3 Pa, and the equilibrium vapor pressure with respect to ice (coi) is 181.2 Pa.
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Scientists are trying to produce a chemical reaction using two substances. After observing the substances, they realize that there is not
enough energy to make the reaction they had hoped for. In this case, what would they need to add to the mixture to produce a quicker
reaction?
O A base
O An acid
O UV light
O A catalyst
Answer: NOT a base
Explanation: i just took the exam and got this one wrong but i donno the correct answer between the last 3 good luck bb
Answer:
A catalyst
Explanation:
Catalysts make reaction faster and speed up the reaction time.
Elemental silicon is oxidized by o2 to give a compound which dissolves in molten na2co3. when this solution is treated with aqueous hydrochloric acid, a precipitate forms. what is the precipitate
Elemental silicon is oxidized by O₂ to give a compound which dissolves in molten Na₂CO₃. when this solution is treated with aqueous hydrochloric acid, a precipitate forms. silica gel is the precipitate.
The compound formed by the oxidation of elemental silicon with O₂ is silicon dioxide (SiO₂), which can dissolve in molten Na₂CO₃ to form sodium silicate (Na₂SiO₃).
When this solution is treated with aqueous hydrochloric acid (HCl), the sodium silicate reacts with the HCl to form a precipitate of silica gel (SiO₂·nH₂O). This reaction is known as the gelatinization of sodium silicate. The sodium chloride (NaCl) formed by the reaction remains in solution.
The silica gel precipitate is often used as a desiccant or drying agent due to its high surface area and ability to adsorb water molecules.
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iughy. hbh. bvjh. bh. bhi
Help please brainliest, I promise
According to the stoichiometry of the given chemical equation,2.66 moles of aluminium oxide are formed when 4 moles of oxygen reacts.
What is stoichiometry?It is the determination of proportions of elements or compounds in a chemical reaction. The related relations are based on law of conservation of mass and law of combining weights and volumes.
Stoichiometry is used in quantitative analysis for measuring concentrations of substances present in the sample.
As 3 moles of oxygen gives 2 moles of aluminium oxide, thus, 4 moles of oxygen will give 4×2/3=2.66 moles.
Thus, 2.66 moles of aluminium oxide are formed when 4 moles of oxygen reacts.
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molecules move in random directions when heated in a heat engine, and because of the lack of uniformity in direction of molecular movement, true or false
The statement "molecules move in random directions when heated in a heat engine, and because of the lack of uniformity in the direction of molecular movement" is true.
When a heat engine is heated, molecules absorb heat energy and their kinetic energy increases. The kinetic energy of molecules causes them to move around. However, this movement is not uniform, and the molecules move in random directions.
A heat engine is a device that converts thermal energy into mechanical energy. Heat engines operate on the principle of thermodynamics.
They work by taking in thermal energy from a high-temperature reservoir, converting some of it into mechanical energy, and then releasing the remaining thermal energy to a low-temperature reservoir.The internal combustion engine in a car, the steam engine in an old locomotive, and the turbine in a power plant are all examples of heat engines. They all convert heat energy into mechanical energy to perform work.
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The position vector for an electron is
r
=(4.8 m)
i
^
−(5.0 m)
j
^
+(6.9 m)
k
^
. Find the magnitude of
r
. Number Units
For the position vector for an electron, the magnitude of r is approximately 9.78 m.
To find the magnitude of the position vector r, we can use the formula for the magnitude of a vector:
|r| = sqrt((x²) + (y²) + (z²))
Given that the components of r are:
r = (4.8 m)i - (5.0 m)j + (6.9 m)k
We can substitute these values into the formula to calculate the magnitude:
|r| = sqrt((4.8 m)² + (-5.0 m)² + (6.9 m)²)
|r| = sqrt(23.04 m² + 25.00 m² + 47.61 m²)
|r| = sqrt(95.65 m²)
|r| ≈ 9.78 m
Therefore, the magnitude of r is approximately 9.78 m.
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Polar covalent bonds allow ______ substances to interact with water. Hydrophilic.
Polar covalent bonds allow hydrophilic substances to interact with water. Polar covalent bonds allow hydrophilic substances to interact with water. In a polar covalent bond, the electrons are unequally shared between the atoms, resulting in partial positive and partial negative charges.
Polar covalent bonds occur when there is an unequal sharing of electrons between atoms, resulting in a partial positive charge on one atom and a partial negative charge on another. This polarity allows polar molecules or ions, such as hydrophilic substances, to form favorable interactions with water molecules through hydrogen bonding, which occurs between the positive hydrogen atoms in water and the negative regions of the polar molecule.
The presence of polar covalent bonds in a substance enables it to be hydrophilic, meaning it has an affinity for water. This interaction is crucial in many biological processes, as water serves as a universal solvent and plays a vital role in the dissolution, transport, and reactions of hydrophilic substances. The polarity of the covalent bonds allows for the formation of hydrogen bonds with water, facilitating the solubility and interaction of hydrophilic substances in aqueous environments.
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what is the difference between condensation and accretion? both describe a process by which particles grow atom by atom, but accretion happens at cold temperatures while condensation occurs at hot temperatures. both describe a process by which particles grow atom by atom, but accretion happens at hot temperatures while condensation occurs at cold temperatures. condensation is the sticking together of solid particles, while during accretion, particles grow an atom or molecule at a time. during condensation, particles grow an atom or molecule at a time, while accretion is the sticking together of solid particles. need help?
Condensation is a process of conversion of the gaseous phase into liquid phase whereas accretion is a process of accumulation of small particles into larger ones over a period of time.
Condensation is the process by which water vapor in the air is changed into liquid water it's generally the opposite of evaporation. Condensation is crucial to the water cycle because it is responsible for the formation of clouds.
Accretion is the accumulation of smaller particles into a massive object by gravitationally pulling more matter towards itself, typically gaseous matter, in an accretion process. Most astronomical objects, such as galaxies, stars, and planets, are formed by the same accretion processes itself.
Accretion from natural causes, that adds soil, sand, and also other types of earth to the part of a person's property that generally borders water. While this occurs very slowly, a piece of property may grow a lot overtime and also may increase its value.
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22.4 liters of CO2 at STP, 0◦C and 1 atm,
contains how many molecules? (Assume CO2
is an ideal gas.)
Answer:
There are \(6.01\times 10^{23}\) number of molecules.
Explanation:
Given that,
Volume, V = 22.4 L
Temperature, T = 0°C = 273.15 K
Pressure, P = 1 atm = 1.013 bar
We need to find the number of molecules. Let there are n number of molecules. We know that, ideal gas law is as follows :
\(PV=nRT\\\\n=\dfrac{PV}{RT}\\\\n=\dfrac{1.013\times 22.4}{0.08 314\times 273.15}\)
n = 0.999 moles
No. of molecules,
\(N=n\times N_A\\\\=0.999\times 6.023\times 10^{23}\\\\N=6.01\times 10^{23}\)
So, there are \(6.01\times 10^{23}\) number of molecules.
can someone help with this please? greatly appreciated!
Answer:
Explanation:
a) When the iron rod is heated, it expands (gets larger). That's the key to this whole procedure. It doesn't expand much -- depending on what temperature the iron rod is heated up to.
When the rod looks like it has expanded as much as it can, the rod is tightened as much as it can be tightened before damage is done. When the rod cools, it contracts (gets smaller). That brings the walls closer together or it stops the walls from bulging.
Very interesting question. I've never seen this. Thanks for asking.
is shaking a can of soda a physical or chemical change?
Answer:
Chemical, the foaming of the ingredients makes it a chemical reaction
Explanation:
hope this helps
What is the main factor that controls the type of volcano that forms
following eruption?
Answer:
the buoyancy of the magma, the pressure from the exsolved gases in the magma and the injection of a new batch of magma into an already filled magma chamber
The buoyancy of the magma is the main factor that controls the type of volcano that forms following eruption.
What is volcano?A volcano is a crack in the crust of the Earth, another planet, or a satellite from which molten rock, hot stone pieces, and hot gases are released in eruptions. An amazing demonstration of Earth's might is a volcanic eruption.
Eruptions may cause catastrophic loss of lives and property, particularly in areas of the planet that are highly inhabited, despite the fact that they are beautiful to observe. The buoyancy of the magma is the main factor that controls the type of volcano that forms following eruption.
Therefore, the buoyancy of the magma is the main factor that controls the type of volcano that forms following eruption.
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2. If I have an unknown volume of gas held at a temperature of 115. K in a container with a pressure of 60.0 kPa with 1.50 mol of particles. If by increasing the temperature to 225. K, decreasing the pressure to 30.0 kPa, and allowing some gas to escape until 1.00 moles remain causes the volume of the gas to be 29.0 liters, how many liters of gas did I start with?
The initial volume of the gas was approximately 14.5 liters. The molar volume of a gas expresses the volume occupied by 1 mole of that respective gas under certain temperature and pressure conditions. This answer supports our expectation from Charles law.
To find the initial volume, we can use the Ideal Gas Law formula, which is PV = nRT. Here, P is the pressure, V is the volume, n is the number of moles, R is the ideal gas constant (8.314 J/mol·K), and T is the temperature in Kelvin. Step 1, Convert the given pressure from kPa to Pa by multiplying by 1000. So, 60.0 kPa * 1000 = 60000 Pa. Step 2, Plug the values into the Ideal Gas Law formula: 60000 Pa * V = 1.50 mol * 8.314 J/mol·K * 115 K. Step 3, Solve for V: V = 1.50 mol * 8.314 J/mol·K * 115 K / 60000 Pa. Step 4, Calculate the volume: V ≈ 14.5 liters.
So, you started with approximately 14.5 liters of gas. Finally, we can use the fact that the initial number of moles of particles was 1.50, and that only 1.00 moles remained after some gas escaped, to calculate the initial volume of the gas, so the correct number is 14.5 liters.
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how come everytime my mom yells at me i feel myself drift more and more away from life
Answer:oml felt
Explanation:
I'm sorry tho
Answer:
800-273-8255
Explanation:
here is the sui cide hotline number
How does the overall charge of oxygen of -16 compare to the overall charge of oxygen -17 and oxygen -18?
Answer:
-2
The charge on an oxygen ion is -2. An oxygen atoms contain six electrons in its valence electron shell.
Explanation:
what unit is a wave velocity measured in
Answer:
radians
Explanation:
Answer:
Meters per second.
Explanation:
Ap3x approved
what are the 2 characteristics of fluids♂️
I think that you should use your phone
Which two changes would make this reaction reactant-favored?
2H₂+02 2H₂O + energy
A. Reducing the pressure
B. Increasing the pressure
C. Reducing the temperature
D. Increasing the temperature
C. Reducing the temperature, is the two changes would make this reaction reactant-favored 2H₂+O₂ 2H₂O + energy
To make the given reaction reactant-favored, we need to shift the equilibrium towards the left side, favoring the formation of reactants (H₂ and O₂) rather than products (H₂O). This can be achieved by considering the impact of pressure and temperature on the reaction.
A. Reducing the pressure:
Reducing the pressure would not favor the reactants. According to Le Chatelier's principle, decreasing the pressure will shift the equilibrium towards the side with a higher number of moles of gas. In this case, both sides of the reaction have the same number of moles of gas (two moles), so reducing the pressure will not have a significant effect.
B. Increasing the pressure:
Increasing the pressure would not favor the reactants either. Again, according to Le Chatelier's principle, increasing the pressure will shift the equilibrium towards the side with fewer moles of gas. As both sides have the same number of moles of gas, changing the pressure will not impact the equilibrium.
C. Reducing the temperature:
Reducing the temperature would favor the reactants. The reaction is exothermic (releases energy), and according to Le Chatelier's principle, decreasing the temperature favors the reaction that produces heat. Therefore, reducing the temperature would shift the equilibrium towards the reactants (H₂ and O₂) side.
D. Increasing the temperature:
Increasing the temperature would not favor the reactants. In an exothermic reaction, increasing the temperature would shift the equilibrium towards the products (H₂O) side to absorb the additional heat.
In conclusion, reducing the temperature (option C) would make the reaction reactant-favored, favoring the formation of H₂ and O₂ rather than H₂O. Therefore, Option C is correct.
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if loratadine and pseudoephedrine sulfate are put into a LLE system. what would be the best organic phases and aqueous phase to separate them?
In a liquid-liquid extraction (LLE) system with loratadine and pseudoephedrine sulfate, the choice of organic and aqueous phases is crucial for efficient separation.
Loratadine is a non-polar compound, while pseudoephedrine sulfate is more polar due to the presence of a sulfate group. To achieve effective separation, we need to choose a suitable organic phase and aqueous phase.
For the organic phase, a non-polar solvent such as diethyl ether, ethyl acetate, or chloroform can be used. These solvents have good solubility for loratadine. The non-polar organic phase will extract loratadine from the mixture, allowing for separation from the aqueous phase.
As for the aqueous phase, we need to choose a polar solvent that will selectively extract pseudoephedrine sulfate. Aqueous solutions of acids like hydrochloric acid (HCl) or sulfuric acid (H2SO4) can be used. The acidic conditions protonate the pseudoephedrine sulfate, making it more soluble in the aqueous phase.
To perform the extraction, the mixture of loratadine and pseudoephedrine sulfate is added to the LLE system consisting of the organic phase (non-polar solvent) and the aqueous phase (acidic solution). The mixture is then vigorously shaken to ensure thorough mixing and extraction.
After shaking, the phases are allowed to separate, with the more dense aqueous phase settling at the bottom and the less dense organic phase on top. The layers can be carefully separated, and each phase can be further processed to isolate the desired compounds.
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I would like to know if question number 3 is rite
The balanced reaction of this chemical reaction is as follows :
\(2HClO_4(aq)\text{ + Ba}(OH)_2(aq)\text{ }\Rightarrow Ba(ClO_4)_2(aq)+2H_2O(l)\text{ }\)• As we can see, this is a double-displament reaction
An alkyne is represented by the molecular formula? a)C6H6
b)C5H12 c)C4H6 d)C3H6
An alkyne is represented by the molecular formula of (d) C3H6.
A chemical compound is represented by a molecular formula. It describes the number and kind of atoms present in a molecule. An alkyne is a type of hydrocarbon. It is a type of unsaturated hydrocarbon having a triple bond between two carbon atoms. Thus, an alkyne is represented by the molecular formula CnH2n-2.
The carbon-carbon triple bond in alkynes is a strong bond that consists of one sigma bond and two pi bonds.
The molecular formula of an alkyne is CnH2n-2. The hydrocarbons with triple bonds have a higher degree of unsaturation, thus they are more reactive than their corresponding alkenes. Alkynes are used in the preparation of various compounds that are used in our daily lives.
Some of the uses of alkynes are:
It is used in welding.
It is used in organic synthesis.
It is used in the production of synthetic rubber.
It is used in the production of plastics such as nylon and neoprene.
Hence, the correct option is (d) C3H6.
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Edg. 2023
Can't find the answer anywhere else
The temperature of the gas is 294.15 K and volume of the gas is 1.55 cm³.
How to calculate gas temperature and volume?To calculate the temperature of gas in Kelvin, add 273.15 to the Celsius temperature:
Temperature of gas: 21°C + 273.15 = 294.15 K
To calculate the volume of the gas using the formula V = πr²h, first calculate the radius using the given information. The radius is given as 5 cm. Therefore:
r = 0.5 cm
The height of the column of gas is given as 6.2 cm. Therefore:
h = 6.2 cm
Now substitute these values into the formula:
V = πr²h
V = π(0.5 cm)²(6.2 cm)
V = π(0.25 cm²)(6.2 cm)
V = 1.55 cm³
Therefore, the volume of the gas at room temperature is approximately 1.55 cm³.
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Answer: 294.15 K and 1.55 cm³
Explanation:
:)
Which of the following is NOT a valid conversion factor?
A. 12 hours/1 days
B. 1 yard/3 feet
C. 1 dozen/12 cookies
D. 60 seconds/1 minute
Answer:
the answer is D I would say thats the answer
Answer:
I really don't know how I got to switching to a new job because I was in a hurry
Which best describes a radioactive isotope?
Answer:
A radioactive isotope, also known as a radioisotope, radionuclide, or radioactive nuclide, is any of several species of the same chemical element with different masses whose nuclei are unstable and dissipate excess energy by spontaneously emitting radiation in the form of alpha, beta, and gamma rays.
Answer:
unstable
Explanation:
A sphere of radius 0.457 m, temperature 32.2 ∘
C, and emissivity 0.924 is located in an environment of temperature 82.9 ∘
C. At what rate does the sphere (a) emit and (b) absorb thermal radiation? (c) What is the sphere's net rate of energy exchange? (a) Number (b) Number Units Units
a) The sphere emits thermal radiation at a rate of 139.75 Watts.
b) The sphere absorbs thermal radiation at a rate of 37.66 Watts.
c) The sphere's net rate of energy exchange is 102.09 Watts.
What are the rates of thermal radiation emission, absorption, and net energy exchange for the sphere?To calculate the rates of thermal radiation emission and absorption, we can use the Stefan-Boltzmann law, which states that the rate of thermal radiation emitted or absorbed by an object is proportional to its surface area, temperature, and the Stefan-Boltzmann constant.
a) The rate of thermal radiation emitted by the sphere can be calculated using the formula:
Emitting Rate = emissivity * surface area * Stefan-Boltzmann constant * (\(temperature^4 - environment\ temperature^4\))
Plugging in the given values:
Emitting Rate = \(0.924 * (4\pi * (0.457)^2) * 5.67 \times 10^{-8} * ((32.2 + 273.15)^4 - (82.9 + 273.15)^4)\)
Emitting Rate ≈ 139.75 Watts
b) The rate of thermal radiation absorbed by the sphere can be calculated in a similar way but using the environment temperature as the object's temperature:
Absorbing Rate = emissivity * surface area * Stefan-Boltzmann constant * (\(environment\ temperature^4 - temperature^4\))
Plugging in the given values:
Absorbing Rate = \(0.924 * (4\pi * (0.457)^2) * 5.67 \times 10^{-8} * ((82.9 + 273.15)^4 - (32.2 + 273.15)^4)\)
Absorbing Rate ≈ 37.66 Watts
c) The net rate of energy exchange is the difference between the emitting rate and the absorbing rate:
Net Rate = Emitting Rate - Absorbing Rate
Net Rate = 139.75 Watts - 37.66 Watts
Net Rate ≈ 102.09 Watts
Therefore, the sphere emits thermal radiation at a rate of 139.75 Watts, absorbs thermal radiation at a rate of 37.66 Watts, and has a net rate of energy exchange of 102.09 Watts.
Note: The units for all the rates are Watts.
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In the future the universe will most likely
Answer:A
Explanation: The universe is always expanding at all times. It dosnt expand that much though, very little
Match the terms to their definition. 1. element one of the starting substances (ingredients) that is involved in a chemical reaction 2. heterogeneous the substance that does the dissolving 3. homogenous a substance that is made from only one type of atom. 4. product composed of dissimilar parts which can be separated easily and which are unevenly distributed in the mixture 5. reactant even distribution of parts throughout the whole mixture; not easily separated into individual components 6. solute the substance that is dissolved 7. solvent the substance created as a result of a chemical reaction
Answer:
1. Element - a substance that is made from only one type of atom
An element is made up of only one type of atoms and known for its unique properties than other elements.
2. Heterogeneous - composed of dissimilar parts which can be separated easily and which are unevenly distributed in the mixture
3.Homogenous - even distribution of parts throughout the whole mixture; not easily separated into individual components.
4. Product - the substance created as a result of a chemical reaction
Products are the final substance that created as a result of a chemical reaction.
5. Reactant - one of the starting substances (ingredients) that is involved in a chemical reaction
Reactants are the starting substance that takes part in a chemical reaction.
6. Solute - the substance that is being dissolved.
A Solute is a substance that is being dissolved in the solvent to make a solution.
7. Solvent - the substance that does the dissolving
A solvent is the medium in which the solute is being dissolved to make the solution.
A homogeneous mixture, like a solution, has an even distribution of its components throughout the whole mixture. It is not easily separated into individual components. On the other hand, a heterogeneous mixture, like a mixture of oil and water, has dissimilar parts that can be separated easily, and the components are unevenly distributed in the mixture.
1. Reactant: one of the starting substances (ingredients) that is involved in a chemical reaction.
2. Solvent: the substance that does the dissolving.
3. Solute: the substance that is dissolved.
4. Homogeneous: even distribution of parts throughout the whole mixture; not easily separated into individual components.
5. Heterogeneous: composed of dissimilar parts which can be separated easily and which are unevenly distributed in the mixture.
6. Element: a substance that is made from only one type of atom.
7. Product: the substance created as a result of a chemical reaction.
Let's match the terms to their definitions:
1. Element: A substance that is made from only one type of atom.
2. Reactant: One of the starting substances (ingredients) that is involved in a chemical reaction.
3. Product: The substance created as a result of a chemical reaction.
4. Solute: The substance that is dissolved.
5. Solvent: The substance that does the dissolving.
6. Homogeneous: Even distribution of parts throughout the whole mixture; not easily separated into individual components.
7. Heterogeneous: Composed of dissimilar parts which can be separated easily and which are unevenly distributed in the mixture.
For example, if we consider the reaction between hydrogen gas (H2) and oxygen gas (O2) to form water (H2O), hydrogen gas and oxygen gas would be the reactants. Water would be the product. In this reaction, hydrogen gas and oxygen gas are the starting substances that undergo a chemical reaction to form water.
In a solution, such as saltwater, the salt (solute) dissolves in the water (solvent). The salt is the substance that is dissolved, and the water is the substance that does the dissolving.
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NEED ANSWER ASAP!! I would really aprecitate it! Please.
Explanation:
Positron..............Why should negatively stained slides be handled with extra precaution?
a. The acidic stain is especially toxic.
b. Heating a slide makes it more susceptible to breakage.
c. The live organisms in the inoculum are not killed with heat fixation.
d. The counterstain used is especially toxic.
Negatively stained slides should be handled with extra precaution because the live organisms in the inoculum are not killed with heat fixation, and the counterstain used is especially toxic. The acidic stain and heating of the slide are not the main reasons for the need of extra caution.
Negatively stained slides are prepared using a technique called negative staining, where the background is stained and the organisms appear as colorless or lightly stained against the dark background. Unlike other staining techniques, negative staining does not involve heat fixation, which typically kills the organisms and makes them safer to handle. Therefore, the live organisms present in the inoculum remain viable on negatively stained slides, posing a potential risk of infection or contamination if proper precautions are not taken. Additionally, the counterstain used in negative staining can be particularly toxic. The counterstain is usually an acidic dye, which helps to create contrast and enhance the visibility of the organisms against the stained background. Acidic dyes can have harmful effects on human health if ingested or if they come into contact with the skin or mucous membranes. Therefore, it is crucial to handle negatively stained slides with extra care to avoid any direct contact with the counterstain and to prevent accidental ingestion or inhalation of the toxic substances. In conclusion, negatively stained slides require additional caution during handling due to the presence of live organisms that are not killed during the staining process and the potential toxicity of the counterstain used. Proper safety measures should be followed, such as wearing appropriate personal protective equipment (PPE) and ensuring proper disposal of the slides after use, to minimize the risk of contamination and exposure to potentially harmful substances.
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